Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   ACLEAH_RS25130 Genome accession   NZ_CP177242
Coordinates   5360594..5361931 (+) Length   445 a.a.
NCBI ID   WP_003110427.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SM45     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5355594..5366931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLEAH_RS25105 (ACLEAH_25105) pgeF 5355737..5356465 (-) 729 WP_009316270.1 peptidoglycan editing factor PgeF -
  ACLEAH_RS25110 (ACLEAH_25110) rluD 5356462..5357424 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACLEAH_RS25115 (ACLEAH_25115) - 5357570..5358595 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  ACLEAH_RS25120 (ACLEAH_25120) - 5358752..5358997 (+) 246 WP_009316269.1 PP0621 family protein -
  ACLEAH_RS25125 (ACLEAH_25125) pilS 5358987..5360579 (+) 1593 WP_003094692.1 two-component system sensor histidine kinase PilS Regulator
  ACLEAH_RS25130 (ACLEAH_25130) pilR 5360594..5361931 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  ACLEAH_RS25135 (ACLEAH_25135) thiO 5361977..5363071 (-) 1095 WP_033995405.1 glycine oxidase ThiO -
  ACLEAH_RS25140 (ACLEAH_25140) - 5363206..5363715 (+) 510 WP_003134932.1 Tfp pilus assembly protein FimT/FimU -
  ACLEAH_RS25145 (ACLEAH_25145) - 5363821..5364327 (+) 507 WP_023098086.1 Tfp pilus assembly protein FimT/FimU -
  ACLEAH_RS25150 (ACLEAH_25150) pilV 5364318..5364875 (+) 558 WP_003110422.1 type 4a pilus minor pilin PilV Machinery gene
  ACLEAH_RS25155 (ACLEAH_25155) pilW 5364872..5365696 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  ACLEAH_RS25160 (ACLEAH_25160) pilX 5365693..5366280 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49723.03 Da        Isoelectric Point: 5.8638

>NTDB_id=1086408 ACLEAH_RS25130 WP_003110427.1 5360594..5361931(+) (pilR) [Pseudomonas aeruginosa strain SM45]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=1086408 ACLEAH_RS25130 WP_003110427.1 5360594..5361931(+) (pilR) [Pseudomonas aeruginosa strain SM45]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGCCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCAGGCAACGTCCGCGAGCTGGAGAATATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGAACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment