Detailed information NTG1085596

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACLBNB_RS27275
• Genome accession: NZ_CP177156
• Coordinates: 5985333..5986205 (+)
• Length: 290 a.a.
• NCBI ID: WP_009050909.1
• Uniprot ID: A0AB33WWI2
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain YL-21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5980333..5991205

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLBNB_RS27260 (ACLBNB_27260)	-	5982688..5983044 (-)	357	WP_009050906.1	BON domain-containing protein	-
ACLBNB_RS27265 (ACLBNB_27265)	-	5983332..5983748 (-)	417	WP_009050907.1	pilin	-
ACLBNB_RS27270 (ACLBNB_27270)	pilC	5984113..5985330 (+)	1218	WP_135006961.1	type II secretion system F family protein	Machinery gene
ACLBNB_RS27275 (ACLBNB_27275)	pilD	5985333..5986205 (+)	873	WP_009050909.1	prepilin peptidase	Machinery gene
ACLBNB_RS27280 (ACLBNB_27280)	coaE	5986202..5986825 (+)	624	WP_151264653.1	dephospho-CoA kinase	-
ACLBNB_RS27285 (ACLBNB_27285)	yacG	5986822..5987025 (+)	204	WP_009045670.1	DNA gyrase inhibitor YacG	-
ACLBNB_RS27290 (ACLBNB_27290)	-	5987030..5987245 (-)	216	WP_009050911.1	hypothetical protein	-
ACLBNB_RS27295 (ACLBNB_27295)	-	5987311..5988000 (-)	690	WP_411835827.1	energy-coupling factor ABC transporter permease	-
ACLBNB_RS27300 (ACLBNB_27300)	-	5987997..5988464 (-)	468	WP_016703672.1	hypothetical protein	-
ACLBNB_RS27305 (ACLBNB_27305)	-	5988919..5990355 (+)	1437	WP_124312167.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32050.22 Da
• Isoelectric Point: 7.9954

>NTDB_id=1085596 ACLBNB_RS27275 WP_009050909.1 5985333..5986205(+) (pilD) [Pseudomonas chlororaphis subsp. aurantiaca strain YL-21]
MSLTEVLASYPLVFVASMLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPVEPAGPTYNLLLPHSQCPHCAHSIR
PWENIPVFSYLLLRGRCSACQAPIGRRYPLVELTCGLASAFVAWHFGFGWQAVLMLLLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGIALLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1085596 ACLBNB_RS27275 WP_009050909.1 5985333..5986205(+) (pilD) [Pseudomonas chlororaphis subsp. aurantiaca strain YL-21]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGATGCTGGTCCTGGGACTGATTGTCGGCAG
CTTTCTCAATGTGCTGATCTGGCGTCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCCCGTGACATCCTGGGGC
TGCCCGTCGAGCCTGCCGGGCCGACCTACAACCTGCTGCTCCCGCACTCCCAGTGCCCTCACTGCGCACATTCGATCCGC
CCCTGGGAAAACATCCCGGTGTTCAGCTACCTGCTGCTGCGTGGACGCTGCTCCGCGTGCCAGGCACCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGTCC
TGATGCTGCTGCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGACCATCAACTGTTGCCCGACGTGCTGGTG
CTGCCGCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTTTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGCGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACAGGA
GCCCTGGCCGGGATCGCCCTGCTGCGCCGCAACCGTGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.817	97.931	0.517
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	50.909	94.828	0.483
pilD	Acinetobacter nosocomialis M2	50.182	94.828	0.476
pilD	Neisseria gonorrhoeae MS11	47.794	93.793	0.448