Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AACH71_RS00065 Genome accession   NZ_AP029050
Coordinates   8156..8773 (+) Length   205 a.a.
NCBI ID   WP_136654233.1    Uniprot ID   -
Organism   Bacillus stercoris strain BST19     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3156..13773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH71_RS00030 (BSB_00040) aroE 3814..4656 (+) 843 WP_338709578.1 shikimate dehydrogenase -
  AACH71_RS00035 (BSB_00050) yhbY 4650..4940 (+) 291 WP_014664674.1 ribosome assembly RNA-binding protein YhbY -
  AACH71_RS00040 (BSB_00060) - 4952..5521 (+) 570 WP_014477393.1 nicotinate-nucleotide adenylyltransferase -
  AACH71_RS00045 (BSB_00070) yqeK 5511..6071 (+) 561 WP_014664672.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AACH71_RS00050 (BSB_00080) rsfS 6089..6445 (+) 357 WP_014664671.1 ribosome silencing factor -
  AACH71_RS00055 (BSB_00090) - 6442..7185 (+) 744 WP_014664670.1 class I SAM-dependent methyltransferase -
  AACH71_RS00060 (BSB_00100) comER 7251..8072 (-) 822 WP_004398597.1 late competence protein ComER -
  AACH71_RS00065 (BSB_00110) comEA 8156..8773 (+) 618 WP_136654233.1 helix-hairpin-helix domain-containing protein Machinery gene
  AACH71_RS00070 (BSB_00120) - 8840..9409 (+) 570 WP_014664668.1 ComE operon protein 2 -
  AACH71_RS00075 (BSB_00130) comEC 9413..11743 (+) 2331 WP_136654232.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AACH71_RS00080 (BSB_00140) - 11784..11918 (-) 135 WP_003229983.1 YqzM family protein -
  AACH71_RS00085 - 11995..12108 (+) 114 WP_134975484.1 hypothetical protein -
  AACH71_RS00090 (BSB_00150) holA 12148..13191 (+) 1044 WP_014664666.1 DNA polymerase III subunit delta -
  AACH71_RS00095 (BSB_00160) rpsT 13206..13472 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21749.57 Da        Isoelectric Point: 4.9616

>NTDB_id=108226 AACH71_RS00065 WP_136654233.1 8156..8773(+) (comEA) [Bacillus stercoris strain BST19]
MNWLNQHKKAMILAASAAVFTAIMIFLASGRNKEPVKQAVPTQTVNTEVKQEAGKDEPSEAIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTNEQADEVQVNLAEILQDGTVVYIPKKGEETAVQQGAGGAVQSGGGKGELVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSISVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=108226 AACH71_RS00065 WP_136654233.1 8156..8773(+) (comEA) [Bacillus stercoris strain BST19]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATGATTTTAGCGGCTTCTGCAGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCAGCGGGAGAAACAAAGAGCCGGTGAAGCAGGCTGTACCAACTCAGACGGTAAATACAGAGGTAAAGCAGGAAGCAG
GCAAAGATGAGCCAAGCGAAGCAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTCTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGTGGAACCAATGAGCAAGCAGATGAAGTGCAAGTGAATTTGGCGGA
GATTCTGCAGGACGGGACAGTAGTGTATATCCCAAAAAAAGGAGAGGAAACAGCAGTGCAACAGGGTGCCGGAGGAGCTG
TTCAAAGCGGTGGAGGGAAGGGGGAGCTGGTAAATATCAATACAGCCACCTTAGAGGAGTTACAAGGCATCTCAGGGGTA
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACTATTGAAGATATCACGAAGGT
TTCAGGAATAGGCGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTTCAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

91.22

100

0.912
  comEA Lactococcus lactis subsp. cremoris KW2

34.956

100

0.385

Multiple sequence alignment