Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH87_RS02710 Genome accession   NZ_AP029019
Coordinates   597998..599134 (-) Length   378 a.a.
NCBI ID   WP_338797183.1    Uniprot ID   -
Organism   Acidovorax sp. DW039     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 592998..604134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH87_RS02685 (ACDW_05300) rsmI 593780..594739 (-) 960 WP_338797178.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  AACH87_RS02690 (ACDW_05310) - 594739..595158 (+) 420 WP_338797179.1 YraN family protein -
  AACH87_RS02695 (ACDW_05320) - 595535..596137 (+) 603 WP_338797180.1 SIS domain-containing protein -
  AACH87_RS02700 (ACDW_05330) - 596134..596793 (+) 660 WP_338797181.1 BON domain-containing protein -
  AACH87_RS02705 (ACDW_05340) - 596897..597811 (-) 915 WP_338797182.1 NAD(P)-dependent oxidoreductase -
  AACH87_RS02710 (ACDW_05350) pilU 597998..599134 (-) 1137 WP_338797183.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACH87_RS02715 (ACDW_05360) - 599200..599835 (-) 636 WP_338797184.1 cyclic nucleotide-binding domain-containing protein -
  AACH87_RS02720 (ACDW_05370) pilT 599900..600943 (-) 1044 WP_338797185.1 type IV pilus twitching motility protein PilT Machinery gene
  AACH87_RS02725 (ACDW_05380) - 600982..601704 (+) 723 WP_338797187.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH87_RS02730 (ACDW_05390) ltaE 601746..602810 (-) 1065 WP_338797188.1 low-specificity L-threonine aldolase -
  AACH87_RS02735 (ACDW_05400) - 602977..604116 (+) 1140 WP_338797189.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41908.31 Da        Isoelectric Point: 6.9660

>NTDB_id=108112 AACH87_RS02710 WP_338797183.1 597998..599134(-) (pilU) [Acidovorax sp. DW039]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTSNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFVQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEITMTKRGLCIMVGATGSGKSTTLAAMVDWRNEHSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGMVSQRLIPKQDGKGRAAAVEVMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQSLFDLYEANIISYEDALRNADSLNDLRLQIKLNSQRAKSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=108112 AACH87_RS02710 WP_338797183.1 597998..599134(-) (pilU) [Acidovorax sp. DW039]
ATGGAACGCGATCAGGCCAGTAAATTCATCAATGACCTGCTCAAGTTGATGGTAAGCCGCAATGGCAGTGACTTGTTCAT
CACAGCAGAGTTTCCGCCGGCTATCAAGGTCGACGGCAAGGTTACCAAGGTATCTCCCCAACCGCTGACCTCCAACCACA
CGCTCACGCTGGCCCGCGCCATCATGAGCGACAAGCAGGTGGCTGATTTTGAGCGCACCAAGGAGTGCAATTTCGCCATC
TCGCCTGCGGGCATTGGCCGCTTCCGGGTCAATGCCTTTGTGCAGCAGGGCAAGGTCGGCATGGTGTTGCGGACCATTCC
GCTCACGCTGCCCACCATTGATGGCTTGGGCGTGCCGCAGGTGCTCAAGGAAATCACGATGACCAAGCGCGGCTTGTGCA
TCATGGTGGGAGCCACCGGCTCGGGTAAATCCACCACGCTGGCAGCCATGGTGGACTGGCGCAATGAGCACTCTTTCGGT
CACATCATCACGGTGGAAGACCCTGTTGAATTCGTGCACCCCCACAAGAACTGCGTGGTGACGCAGCGTGAGGTGGGGCT
GGACACTGACAGCTGGGAAGCTGCACTCAAGAACACGCTGCGACAGGCACCCGATGTGATCCTGATGGGCGAGATCCGTG
ACCGCGAAACCATGGAACATGCCGTGGCTTTTGCGGAAACAGGGCACTTGTGTCTTGCCACGCTGCATGCCAACAGTGCC
AACCAAGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGGCGTGCCCAGTTGCTGATGGATCTGTCGCTGAACCT
GCGCGGCATGGTGTCGCAGCGCCTCATCCCCAAGCAGGATGGCAAAGGCCGTGCCGCTGCGGTGGAGGTCATGCTCAACA
CCCCGCTGATCTCTGATCTGATCTTCAAGGGCGAAGTCTCCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACTTTTGACCAGTCGCTCTTTGACCTGTACGAGGCCAACATCATCAGCTATGAAGATGCGCTGCGCAACGCCGA
TTCGCTGAACGATCTGCGCCTGCAGATCAAGCTCAACAGCCAACGCGCCAAGTCACCTGATCTGGCATCGGGTACCGAGC
ATTTCGCCATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

58.543

94.444

0.553
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368

Multiple sequence alignment