Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACKZ7S_RS08000 Genome accession   NZ_CP176565
Coordinates   1630841..1631338 (-) Length   165 a.a.
NCBI ID   WP_115211242.1    Uniprot ID   -
Organism   Staphylococcus aureus strain V0353     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1625841..1636338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZ7S_RS07975 (ACKZ7S_07975) gcvPB 1626003..1627475 (-) 1473 WP_162636190.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACKZ7S_RS07980 (ACKZ7S_07980) gcvPA 1627468..1628814 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACKZ7S_RS07985 (ACKZ7S_07985) gcvT 1628834..1629925 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACKZ7S_RS07990 (ACKZ7S_07990) - 1630084..1630608 (-) 525 WP_162636188.1 shikimate kinase -
  ACKZ7S_RS07995 (ACKZ7S_07995) - 1630598..1630744 (-) 147 WP_001789879.1 hypothetical protein -
  ACKZ7S_RS08000 (ACKZ7S_08000) comGF 1630841..1631338 (-) 498 WP_115211242.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACKZ7S_RS08005 (ACKZ7S_08005) comGE 1631256..1631555 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACKZ7S_RS08010 (ACKZ7S_08010) comGD 1631542..1631988 (-) 447 WP_162635820.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACKZ7S_RS08015 (ACKZ7S_08015) comGC 1631966..1632277 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKZ7S_RS08020 (ACKZ7S_08020) comGB 1632291..1633361 (-) 1071 WP_000776417.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACKZ7S_RS08025 (ACKZ7S_08025) comGA 1633333..1634307 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKZ7S_RS08030 (ACKZ7S_08030) - 1634359..1634982 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACKZ7S_RS08035 (ACKZ7S_08035) - 1634979..1635308 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACKZ7S_RS08040 (ACKZ7S_08040) - 1635308..1636294 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19228.04 Da        Isoelectric Point: 10.2192

>NTDB_id=1080901 ACKZ7S_RS08000 WP_115211242.1 1630841..1631338(-) (comGF) [Staphylococcus aureus strain V0353]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLVEMLIAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNMQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1080901 ACKZ7S_RS08000 WP_115211242.1 1630841..1631338(-) (comGF) [Staphylococcus aureus strain V0353]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTGTTGAAATGTTAATAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTTATTGAAAGTAGGGAATTAACAACTGTAGATTTTGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATATGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment