Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACKZ7S_RS08025 Genome accession   NZ_CP176565
Coordinates   1633333..1634307 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain V0353     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1628333..1639307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZ7S_RS07985 (ACKZ7S_07985) gcvT 1628834..1629925 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACKZ7S_RS07990 (ACKZ7S_07990) - 1630084..1630608 (-) 525 WP_162636188.1 shikimate kinase -
  ACKZ7S_RS07995 (ACKZ7S_07995) - 1630598..1630744 (-) 147 WP_001789879.1 hypothetical protein -
  ACKZ7S_RS08000 (ACKZ7S_08000) comGF 1630841..1631338 (-) 498 WP_115211242.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACKZ7S_RS08005 (ACKZ7S_08005) comGE 1631256..1631555 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACKZ7S_RS08010 (ACKZ7S_08010) comGD 1631542..1631988 (-) 447 WP_162635820.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACKZ7S_RS08015 (ACKZ7S_08015) comGC 1631966..1632277 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKZ7S_RS08020 (ACKZ7S_08020) comGB 1632291..1633361 (-) 1071 WP_000776417.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACKZ7S_RS08025 (ACKZ7S_08025) comGA 1633333..1634307 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKZ7S_RS08030 (ACKZ7S_08030) - 1634359..1634982 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACKZ7S_RS08035 (ACKZ7S_08035) - 1634979..1635308 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACKZ7S_RS08040 (ACKZ7S_08040) - 1635308..1636294 (-) 987 WP_000161315.1 ROK family glucokinase -
  ACKZ7S_RS08045 (ACKZ7S_08045) - 1636291..1636494 (-) 204 WP_162635819.1 YqgQ family protein -
  ACKZ7S_RS08050 (ACKZ7S_08050) - 1636475..1637938 (-) 1464 WP_001017867.1 rhomboid family protein -
  ACKZ7S_RS08055 (ACKZ7S_08055) - 1637950..1638489 (-) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  ACKZ7S_RS08060 (ACKZ7S_08060) rpmG 1638698..1638847 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=1080906 ACKZ7S_RS08025 WP_000697220.1 1633333..1634307(-) (comGA) [Staphylococcus aureus strain V0353]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1080906 ACKZ7S_RS08025 WP_000697220.1 1633333..1634307(-) (comGA) [Staphylococcus aureus strain V0353]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment