Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACK1U3_RS08125 Genome accession   NZ_CP176537
Coordinates   1805701..1807920 (+) Length   739 a.a.
NCBI ID   WP_409488948.1    Uniprot ID   -
Organism   Pseudomonas promysalinigenes strain KZH.chiA     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1800701..1812920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK1U3_RS08110 (ACK1U3_08110) - 1803048..1804316 (-) 1269 WP_060479883.1 malic enzyme-like NAD(P)-binding protein -
  ACK1U3_RS08115 (ACK1U3_08115) - 1804485..1805315 (-) 831 WP_409488947.1 thermonuclease family protein -
  ACK1U3_RS08120 (ACK1U3_08120) rpmE 1805318..1805533 (-) 216 WP_054884221.1 50S ribosomal protein L31 -
  ACK1U3_RS08125 (ACK1U3_08125) priA 1805701..1807920 (+) 2220 WP_409488948.1 primosomal protein N' Machinery gene
  ACK1U3_RS08130 (ACK1U3_08130) argS 1808029..1809765 (+) 1737 WP_409488949.1 arginine--tRNA ligase -
  ACK1U3_RS08135 (ACK1U3_08135) - 1809767..1810468 (+) 702 WP_207937374.1 SPOR domain-containing protein -
  ACK1U3_RS08140 (ACK1U3_08140) - 1810521..1810940 (-) 420 WP_060479878.1 NINE protein -
  ACK1U3_RS08145 (ACK1U3_08145) - 1811107..1811736 (+) 630 WP_186476262.1 C40 family peptidase -
  ACK1U3_RS08150 (ACK1U3_08150) pilT 1811811..1812809 (-) 999 WP_409488950.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82388.87 Da        Isoelectric Point: 8.3056

>NTDB_id=1080828 ACK1U3_RS08125 WP_409488948.1 1805701..1807920(+) (priA) [Pseudomonas promysalinigenes strain KZH.chiA]
MPDVILRLALPSPLRRLFDYKAPASMARQALTPGMRVRVPFGRREMIGVLVEVCAQSEVPADKLKPASALLDSVSPIPPA
LFKLCLWTAQYYQHSFGDTLSWALPALLRQGEPAEMRQERFWHVAPDARPEDPRIARAPRQRDALKTLAQHPHGVAHSLL
SKLNLNKDSLDLLLAKELVQLEVRRHQPAPRHEHLLAQPELPLNDEQRDAFDAVREGFDAFAAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRAQQENIPILLGSATPSLETLHNALTGRYRLLRMNQRAGGARPPRMLRLDVKS
LPLDSGISGPLQQAIRQTLEAGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRSGVLRCHHCGYDERLPQQC
PQCNHVDLRPVGAGTERAEERLKVLFPDYPILRVDRDSTARKDAMHTLFTTIQRGQPSILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVVIQTHLTDHPLLVQLTEQGYFAFAEQALDERRNAGLPPFSHL
ALLRAEAHKPGQAEGFLDQACAAAERLVAEQQLPGIELLGPVPAPMERRAGRFRAQLLVQANTRAPLHRLISAWLLVLEQ
LPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1080828 ACK1U3_RS08125 WP_409488948.1 1805701..1807920(+) (priA) [Pseudomonas promysalinigenes strain KZH.chiA]
GTGCCCGACGTCATTCTGCGCCTTGCCCTGCCCTCCCCGTTGCGTCGCCTGTTCGATTACAAGGCGCCTGCAAGCATGGC
GCGCCAAGCACTGACCCCCGGCATGCGCGTACGCGTGCCATTCGGGCGTCGCGAAATGATCGGTGTGCTGGTGGAGGTGT
GTGCACAGAGCGAGGTGCCCGCCGACAAGCTAAAGCCCGCCAGTGCCTTGCTCGACTCGGTTTCGCCGATCCCGCCAGCG
CTGTTCAAGCTGTGCCTATGGACCGCGCAGTATTACCAGCACAGCTTTGGCGACACTTTGAGCTGGGCCCTGCCTGCGCT
GCTGCGCCAGGGCGAGCCCGCCGAGATGCGCCAGGAGCGCTTCTGGCACGTAGCACCCGACGCACGCCCGGAGGACCCAC
GCATCGCCCGCGCGCCCCGTCAGCGCGATGCCCTCAAGACCCTGGCCCAGCACCCGCACGGCGTGGCGCACAGCCTGCTG
AGCAAGCTGAACCTGAATAAGGACAGCCTCGACCTGCTGCTGGCCAAGGAGCTGGTTCAACTTGAGGTACGCCGCCACCA
GCCGGCGCCACGCCATGAACATTTGCTGGCGCAGCCAGAATTACCGCTGAACGACGAACAACGTGATGCGTTCGACGCCG
TGCGGGAGGGCTTTGATGCGTTTGCTGCCTTCCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACCGAGGTGTACCTGCAA
CTGATTCGCGAAACCTTGGAGGCCGGTAAGCAGGCCCTGGTACTGATCCCGGAAATCAACCTAGGGCCGCAAACCCTGGC
GCGCTTCGAGCAGCGCTTCAACGCCCGCATCGCCTTGCTGCACTCGGCCGTGAATGATCGTGAGCGCCTCGACGCCTGGC
TGGCCGCTCGCGACGGCGAGGCCGACATCATCATCGGTACGCGCTCGGCGCTGTTCACGCCGATGAAAAACCCAGGGCTG
ATCATCATCGATGAAGAACACGACGGCTCCTACAAACAGCAGGAAGGCCTGCGCTATCACGCCCGCGACCTGGCGCTGGT
ACGCGCCCAGCAAGAGAACATTCCGATCTTGCTCGGCTCCGCGACGCCGTCGCTGGAAACCTTGCACAACGCACTTACCG
GCCGCTATCGCCTGCTGCGCATGAACCAGCGCGCCGGTGGTGCCCGCCCGCCGCGCATGTTGCGCCTGGATGTGAAGAGC
CTGCCGTTGGACAGCGGTATCAGCGGCCCTTTGCAACAGGCCATTCGCCAGACCCTGGAGGCCGGTCAGCAGGTGCTGGT
GTTCCTCAACCGCCGCGGCTTCGCCCCAACCTTGCTGTGCCATGACTGCGGCTGGCTGTCCGAATGCCCGCGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCTGGCGTGCTGCGTTGCCATCACTGCGGCTACGACGAGCGCCTGCCGCAGCAGTGC
CCGCAGTGCAATCATGTCGACCTGCGCCCGGTTGGCGCCGGCACCGAACGCGCAGAAGAACGCTTGAAAGTGCTTTTTCC
GGACTATCCGATTTTGCGCGTGGACCGCGACAGCACGGCCCGCAAGGACGCCATGCATACGCTTTTCACGACCATTCAGC
GCGGCCAGCCGAGCATCCTCGTGGGCACCCAGATGCTAGCCAAAGGGCATCACTTCCCTCGGGTGACCTTGGTAGCCATC
CTCGATGCCGATGGCGGCCTGTTCTCCGGCGACTTCCGCGCCAGCGAACGCATGGCCCAACTGATCGTGCAGGTAGCGGG
CAGGGCCGGGCGCGCCGAGGAACCGGGCAAGGTGGTCATCCAGACGCACTTGACCGACCACCCACTGCTGGTGCAATTGA
CCGAACAAGGCTACTTCGCCTTCGCCGAACAGGCTTTGGACGAACGGCGCAATGCTGGGCTGCCGCCGTTCTCTCACCTG
GCCCTGCTGCGCGCCGAAGCGCACAAGCCCGGCCAGGCCGAAGGCTTCCTCGACCAAGCCTGCGCCGCGGCCGAGCGCCT
GGTGGCAGAACAGCAACTGCCCGGCATCGAGCTGCTTGGCCCGGTGCCTGCGCCCATGGAGCGGCGCGCCGGGCGCTTTA
GGGCGCAACTGTTGGTTCAGGCCAACACCCGAGCCCCCTTGCATCGACTGATCAGTGCCTGGTTGCTAGTGTTGGAGCAA
CTGCCGAGCGGGCGCCAGGTACGCTGGTCGCTGGACGTCGACCCGGTGGACCTTTATTGA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(206-368)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.715

100

0.48

Multiple sequence alignment