Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACK2WG_RS17985 Genome accession   NZ_CP176536
Coordinates   3508700..3510082 (-) Length   460 a.a.
NCBI ID   WP_326229049.1    Uniprot ID   -
Organism   Bacillus spizizenii strain 29-4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3503700..3515082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS17950 (ACK2WG_17950) flgL 3503866..3504762 (-) 897 WP_014115346.1 flagellar hook-associated protein FlgL -
  ACK2WG_RS17955 (ACK2WG_17955) flgK 3504772..3506295 (-) 1524 WP_409509229.1 flagellar hook-associated protein FlgK -
  ACK2WG_RS17960 (ACK2WG_17960) flgN 3506314..3506796 (-) 483 WP_014115348.1 flagellar protein FlgN -
  ACK2WG_RS17965 (ACK2WG_17965) flgM 3506811..3507077 (-) 267 WP_014115349.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACK2WG_RS17970 (ACK2WG_17970) - 3507157..3507576 (-) 420 WP_079288824.1 TIGR03826 family flagellar region protein -
  ACK2WG_RS17975 (ACK2WG_17975) comFC 3507649..3508371 (-) 723 WP_061186927.1 ComF family protein Machinery gene
  ACK2WG_RS17980 (ACK2WG_17980) - 3508335..3508631 (-) 297 WP_014115352.1 late competence development ComFB family protein -
  ACK2WG_RS17985 (ACK2WG_17985) comFA 3508700..3510082 (-) 1383 WP_326229049.1 ATP-dependent helicase ComFA Machinery gene
  ACK2WG_RS17990 (ACK2WG_17990) - 3510190..3511035 (-) 846 WP_014115354.1 DegV family protein -
  ACK2WG_RS17995 (ACK2WG_17995) degU 3511133..3511822 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACK2WG_RS18000 (ACK2WG_18000) degS 3511905..3513062 (-) 1158 WP_014115355.1 two-component sensor histidine kinase DegS Regulator
  ACK2WG_RS18005 (ACK2WG_18005) - 3513278..3513931 (+) 654 WP_061186930.1 YigZ family protein -
  ACK2WG_RS18010 (ACK2WG_18010) - 3513931..3515055 (+) 1125 WP_061186931.1 LCP family protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52059.22 Da        Isoelectric Point: 9.7037

>NTDB_id=1080809 ACK2WG_RS17985 WP_326229049.1 3508700..3510082(-) (comFA) [Bacillus spizizenii strain 29-4]
MIVPAEKNISFSKELQQTLQSRHLLRTELPFSDKLIEWHIENGYVAAQNSISINKRGYKCNRCGQNDQRYFSFYLSSGKN
QLYCRSCVMMGRVSENVPLYSWIEEDEPNWPQIKLTWDGKLSSGQQKAANVLIDAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQCADISALYGGSDDKGCLSPLMISTTHQLLRYKGAFDVIIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPQELKRKTLNGQLYSVRIPARHHRKPLPEPRFLWCGNWKKKLYRNKIPLAVKR
WIEFHVKEGRPVFLFVPSVTVLEKAVACFKDVNCRAAAVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTIPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEHADGDVIFFHYGKTKSMLDARKHIKEMNALAAKNE

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1080809 ACK2WG_RS17985 WP_326229049.1 3508700..3510082(-) (comFA) [Bacillus spizizenii strain 29-4]
CTGATTGTGCCAGCAGAAAAGAACATTTCTTTTTCAAAAGAATTGCAGCAGACGCTTCAAAGCCGTCATTTGCTCAGAAC
CGAACTCCCATTTTCCGATAAGTTGATTGAATGGCATATCGAAAATGGATATGTCGCTGCCCAAAACTCTATATCCATAA
ATAAACGGGGATATAAGTGTAATAGGTGCGGCCAAAATGATCAGCGGTATTTTTCTTTTTATCTCTCATCTGGAAAGAAT
CAGCTATATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGAATGTTCCTTTATATTCGTGGATAGAGGAGGATGA
ACCAAACTGGCCGCAAATAAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAACAAAAAGCCGCCAATGTCTTAATTG
ATGCAATATCAAAAAAAGAAGAGCTCCTCATTTGGGCTGTTTGCGGCGCTGGCAAAACAGAGATGTTATTCCCCGGTATA
GAATCCGCGTTAAATCAAGGGTTGCGTGTATGTATTGCGACACCCCGCACCGATGTAGTATTAGAGCTTGCTCCTAGACT
CAAGGCTGCCTTTCAGTGTGCTGACATTTCAGCACTTTACGGAGGAAGTGATGACAAAGGCTGTTTATCTCCGCTTATGA
TTTCTACCACACATCAGCTTTTGCGATATAAAGGGGCATTCGACGTTATAATCATTGATGAAGTTGATGCGTTTCCGTAT
TCTGCTGATCAAACACTTCAGTTCGCTGTTCAAAAAGCTAGAAAGAAAAACAGCACCCTTGTTTATTTAAGTGCAACACC
TCCTCAAGAATTAAAAAGAAAAACACTGAATGGACAGTTATATTCAGTCCGTATTCCAGCAAGACACCATCGGAAACCTT
TGCCCGAACCGCGCTTTCTATGGTGTGGAAACTGGAAAAAGAAATTATACCGAAATAAAATCCCGCTAGCGGTAAAAAGA
TGGATAGAGTTTCATGTGAAAGAAGGGAGGCCTGTTTTTTTGTTTGTTCCCTCCGTTACTGTTCTGGAAAAGGCAGTTGC
ATGCTTTAAAGATGTTAATTGCCGAGCAGCGGCAGTGCATGCGGAAGATAAACACAGAAAAGAGAAAGTACAGCAATTCA
GAGACGGTCAGCTTGATCTCTTAATCACAACAACAATATTAGAAAGAGGCGTCACGATTCCCAAGGTGCAAACGGGTGTA
TTAGGGGCGGAATCGCCTATCTTTACGGAAAGCGCACTTGTTCAAATCGCAGGAAGAACCGGGCGGCATAAAGAGCATGC
AGACGGTGATGTCATTTTCTTTCACTACGGAAAAACAAAGAGTATGCTGGATGCAAGAAAGCATATAAAAGAAATGAATG
CCTTGGCAGCAAAAAATGAATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

89.565

100

0.896

Multiple sequence alignment