Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdL   Type   Machinery gene
Locus tag   ACKUB0_RS03850 Genome accession   NZ_CP176093
Coordinates   777158..779056 (-) Length   632 a.a.
NCBI ID   WP_109969648.1    Uniprot ID   A0A2V2MT24
Organism   Methanospirillum lacunae strain Ki8-1     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772158..784056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUB0_RS03835 (ACKUB0_03835) - 773198..775279 (+) 2082 WP_109969652.1 CHASE4 domain-containing protein -
  ACKUB0_RS03840 (ACKUB0_03840) epdK 775229..776233 (-) 1005 WP_109969651.1 type II secretion system F family protein Machinery gene
  ACKUB0_RS03845 (ACKUB0_03845) epdJ 776236..777171 (-) 936 WP_109969649.1 type II secretion system F family protein Machinery gene
  ACKUB0_RS03850 (ACKUB0_03850) epdL 777158..779056 (-) 1899 WP_109969648.1 type II/IV secretion system ATPase subunit Machinery gene
  ACKUB0_RS03855 (ACKUB0_03855) - 779217..779834 (+) 618 WP_109969647.1 hypothetical protein -
  ACKUB0_RS03860 (ACKUB0_03860) - 779866..780222 (-) 357 WP_109969646.1 hypothetical protein -
  ACKUB0_RS03865 (ACKUB0_03865) - 780226..780780 (-) 555 WP_109969645.1 aldolase -
  ACKUB0_RS03870 (ACKUB0_03870) npdG 780799..781449 (-) 651 WP_109969644.1 NADPH-dependent F420 reductase -
  ACKUB0_RS03875 (ACKUB0_03875) - 781451..781723 (-) 273 WP_109969643.1 co-chaperone YbbN -
  ACKUB0_RS03880 (ACKUB0_03880) - 781779..783233 (-) 1455 WP_109969642.1 preprotein translocase subunit SecD -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 71533.61 Da        Isoelectric Point: 5.9206

>NTDB_id=1079657 ACKUB0_RS03850 WP_109969648.1 777158..779056(-) (epdL) [Methanospirillum lacunae strain Ki8-1]
MALKIKFGKGKPKSGGESQSQDSTSVSSDISIKKPRLISSLKKTESGSDKSDITSQLTPEPVKAGIKKFPGVKRVKPSED
VGMQDANLSGSLNPEKGEEKSKKIGFFRKFWSIAPQKRREVYNPDLHGSVVDLTYIPPPGVEEIEVYPVNPPFAYIRITY
NYPNHEYLYEVVEPLYTDSEKKLLDELKKGIFERVNVNTRQISREIAAELLEEMMNEVFADYAIHLDPRTYAKIHYQLHK
DFLGNGMVDAIMHDPLIEDISCDGIERYLFVYHSKYESMETNLKYLDPGDLDSFVTKLAQRAGKHISVAEPMLDATMSDG
SRIQMTLGTEVTAHGSTFTIRKFKEVPITPTDLVEWGTFSPLSIAFLWLAVENGKSCIFAGGTASGKTTSLNAISLFIPP
LAKIVTLEDTRELKLPHANWIPSVTRESFDIAGKGEIDMYELLKAALRQRPEYLIVGEVRGKEALTLFQAMSTGHVTYST
MHADSVASAVHRLENPPISVPRNMLSALDLICVQVQARVGGQRIRRNKQIIEILDIDPRTNELITNEVFRWRQATDEITY
SGKSYILEEIMEAHGWDEERISRDLMQRQEVLEWMRQHDIRDYREVSNIVVSYFRDSEGLMEQIRSGANELI

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=1079657 ACKUB0_RS03850 WP_109969648.1 777158..779056(-) (epdL) [Methanospirillum lacunae strain Ki8-1]
ATGGCGCTCAAGATCAAATTTGGCAAAGGAAAACCAAAGTCTGGTGGAGAGAGCCAAAGCCAGGACTCCACGTCTGTATC
CTCCGATATTTCCATAAAAAAGCCACGGCTCATTTCGAGCCTTAAAAAGACCGAATCAGGATCAGATAAATCAGATATTA
CATCTCAACTAACTCCTGAACCAGTGAAGGCAGGTATCAAAAAGTTTCCTGGAGTGAAAAGAGTAAAACCCTCCGAAGAT
GTAGGGATGCAAGATGCTAATCTGTCCGGTTCATTGAATCCAGAGAAGGGTGAGGAAAAATCTAAAAAAATCGGATTTTT
CAGAAAATTTTGGAGTATCGCTCCCCAAAAGCGAAGAGAGGTATATAATCCTGATCTACATGGTTCTGTCGTTGATCTTA
CCTATATTCCACCACCGGGAGTAGAGGAGATAGAGGTATACCCTGTCAATCCCCCTTTTGCATACATCAGGATAACTTAC
AATTACCCAAACCATGAGTATCTCTACGAGGTTGTAGAACCCCTTTACACAGATTCAGAGAAGAAACTGCTTGATGAGTT
GAAAAAGGGGATCTTTGAACGGGTGAATGTGAACACAAGACAGATCTCCCGTGAGATAGCTGCAGAGCTCCTTGAAGAGA
TGATGAATGAGGTCTTTGCAGATTATGCGATTCACCTGGATCCAAGAACTTATGCCAAGATCCATTATCAACTTCACAAG
GATTTCCTTGGTAACGGTATGGTTGATGCAATCATGCATGACCCGTTGATTGAGGATATCTCATGTGATGGAATCGAGCG
GTATCTCTTTGTGTATCATTCAAAATACGAGTCGATGGAGACGAACCTCAAGTACCTGGATCCTGGTGATCTTGACTCAT
TTGTCACTAAACTAGCACAGCGTGCCGGAAAGCACATCTCTGTCGCTGAGCCGATGCTTGACGCTACAATGTCTGATGGT
TCACGTATCCAGATGACTCTCGGAACTGAAGTCACTGCTCATGGTTCGACCTTTACCATCCGTAAGTTCAAAGAAGTCCC
CATCACGCCGACTGACCTTGTGGAATGGGGTACCTTTTCACCTCTCTCAATAGCTTTCCTCTGGCTTGCAGTGGAGAACG
GGAAATCCTGTATTTTTGCCGGTGGGACCGCATCGGGAAAGACGACCTCACTAAATGCCATCTCCCTTTTTATCCCGCCT
CTGGCAAAGATTGTTACTCTTGAAGATACCCGAGAACTCAAGCTTCCTCATGCAAACTGGATTCCAAGTGTTACCAGGGA
GTCGTTTGATATTGCAGGGAAAGGGGAGATCGATATGTATGAACTCCTCAAGGCCGCCCTTCGTCAGCGTCCAGAGTATC
TGATTGTCGGAGAAGTCCGTGGCAAAGAGGCTCTCACACTCTTTCAGGCAATGAGCACCGGACATGTTACATATTCAACG
ATGCATGCTGATTCAGTTGCCAGTGCGGTTCATCGTTTAGAAAATCCCCCTATTTCTGTGCCCAGGAACATGCTCTCGGC
CTTGGATCTTATTTGTGTGCAGGTCCAGGCAAGGGTCGGTGGGCAACGGATCAGGCGTAACAAGCAGATCATTGAAATCC
TGGATATTGATCCCAGGACTAATGAACTTATCACGAATGAGGTCTTCCGGTGGCGTCAGGCGACTGATGAGATCACATAT
TCAGGAAAATCATACATCCTTGAAGAGATCATGGAAGCTCATGGATGGGACGAAGAGCGGATTAGTCGTGATCTGATGCA
GCGTCAGGAGGTACTTGAATGGATGCGTCAGCATGATATTCGCGATTACCGGGAAGTGAGTAATATTGTTGTCTCGTATT
TCAGGGACTCTGAAGGGCTGATGGAACAGATTCGGTCCGGTGCCAATGAACTCATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(250-507)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V2MT24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdL Methanoculleus thermophilus strain DSM 2373

70.611

82.911

0.585

Multiple sequence alignment