Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdJ   Type   Machinery gene
Locus tag   ACKUB0_RS03845 Genome accession   NZ_CP176093
Coordinates   776236..777171 (-) Length   311 a.a.
NCBI ID   WP_109969649.1    Uniprot ID   A0A2V2MWE8
Organism   Methanospirillum lacunae strain Ki8-1     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 771236..782171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUB0_RS03830 (ACKUB0_03830) - 771628..773079 (+) 1452 WP_109969653.1 response regulator -
  ACKUB0_RS03835 (ACKUB0_03835) - 773198..775279 (+) 2082 WP_109969652.1 CHASE4 domain-containing protein -
  ACKUB0_RS03840 (ACKUB0_03840) epdK 775229..776233 (-) 1005 WP_109969651.1 type II secretion system F family protein Machinery gene
  ACKUB0_RS03845 (ACKUB0_03845) epdJ 776236..777171 (-) 936 WP_109969649.1 type II secretion system F family protein Machinery gene
  ACKUB0_RS03850 (ACKUB0_03850) epdL 777158..779056 (-) 1899 WP_109969648.1 type II/IV secretion system ATPase subunit Machinery gene
  ACKUB0_RS03855 (ACKUB0_03855) - 779217..779834 (+) 618 WP_109969647.1 hypothetical protein -
  ACKUB0_RS03860 (ACKUB0_03860) - 779866..780222 (-) 357 WP_109969646.1 hypothetical protein -
  ACKUB0_RS03865 (ACKUB0_03865) - 780226..780780 (-) 555 WP_109969645.1 aldolase -
  ACKUB0_RS03870 (ACKUB0_03870) npdG 780799..781449 (-) 651 WP_109969644.1 NADPH-dependent F420 reductase -
  ACKUB0_RS03875 (ACKUB0_03875) - 781451..781723 (-) 273 WP_109969643.1 co-chaperone YbbN -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 34248.82 Da        Isoelectric Point: 4.8619

>NTDB_id=1079656 ACKUB0_RS03845 WP_109969649.1 776236..777171(-) (epdJ) [Methanospirillum lacunae strain Ki8-1]
MNSYERFCFNLLGSRMLSSREKYFDLIANLRSARMGITFEAYLATAVITSAIAGFFGSILVALIVYLLNIPALITFKGNI
PQDIASGINQFSVYSLVAGTIIAGIVSFITFGGFTYLIYLIYPSIVAGSRRRDIESSLPYAINYLAAMSTAGIPPAEVFR
QLGSSTIYGESSVEARYVSLEIDVFGKDLIDALKTVSMATPSQRMREFMQGAIGSVASGSNISDYFRTKAEQYTYENRQT
QKQFLDTLALISESYVTALVAGTLFLILIQSVMSILSGEGTPFFLYIIIYLIIPFGSVMFVVMIDAMTPEW

Nucleotide


Download         Length: 936 bp        

>NTDB_id=1079656 ACKUB0_RS03845 WP_109969649.1 776236..777171(-) (epdJ) [Methanospirillum lacunae strain Ki8-1]
ATGAACTCATATGAACGATTCTGCTTCAACCTGCTTGGGAGCAGGATGCTTTCCAGTCGTGAAAAGTATTTTGATCTGAT
CGCAAATCTCCGTTCTGCCCGAATGGGAATTACTTTTGAGGCCTACCTCGCAACAGCAGTTATTACTTCCGCAATTGCTG
GATTCTTCGGTTCCATTCTGGTAGCACTGATTGTATACCTGCTCAATATTCCTGCGTTGATAACGTTCAAAGGAAATATA
CCTCAGGACATTGCTTCTGGTATCAACCAGTTCTCTGTCTATTCCCTCGTTGCTGGAACGATAATCGCAGGTATCGTTTC
CTTCATCACCTTTGGAGGGTTTACCTATTTGATCTACCTCATCTACCCGTCAATTGTAGCAGGCTCAAGGCGGAGAGATA
TCGAGTCAAGCCTGCCATATGCGATCAACTATCTTGCAGCAATGTCTACTGCCGGAATTCCTCCCGCAGAGGTCTTCAGG
CAACTGGGCAGTTCAACAATATATGGTGAGTCTTCTGTTGAGGCCAGGTATGTCTCGCTCGAAATTGATGTATTTGGAAA
AGACCTCATAGATGCTCTCAAAACTGTCTCCATGGCGACCCCGAGCCAAAGAATGAGGGAGTTCATGCAGGGAGCGATTG
GAAGTGTGGCTTCTGGATCTAACATTTCTGATTATTTCAGAACAAAAGCAGAACAGTACACATATGAGAACCGGCAAACA
CAGAAACAATTCCTTGATACCCTTGCACTCATCTCTGAAAGTTATGTAACAGCCCTTGTCGCAGGGACTCTCTTCCTTAT
TCTGATCCAATCTGTCATGTCGATTCTTTCGGGGGAAGGAACCCCGTTCTTTCTCTATATCATCATTTACCTGATCATTC
CGTTTGGGAGTGTGATGTTTGTGGTCATGATTGATGCTATGACACCGGAGTGGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(142-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V2MWE8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdJ Methanoculleus thermophilus strain DSM 2373

60.968

99.678

0.608

Multiple sequence alignment