Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdK   Type   Machinery gene
Locus tag   ACKUB0_RS03840 Genome accession   NZ_CP176093
Coordinates   775229..776233 (-) Length   334 a.a.
NCBI ID   WP_109969651.1    Uniprot ID   A0A2V2N4X5
Organism   Methanospirillum lacunae strain Ki8-1     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 770229..781233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUB0_RS03825 (ACKUB0_03825) - 770257..771474 (+) 1218 WP_109969655.1 DUF4114 domain-containing protein -
  ACKUB0_RS03830 (ACKUB0_03830) - 771628..773079 (+) 1452 WP_109969653.1 response regulator -
  ACKUB0_RS03835 (ACKUB0_03835) - 773198..775279 (+) 2082 WP_109969652.1 CHASE4 domain-containing protein -
  ACKUB0_RS03840 (ACKUB0_03840) epdK 775229..776233 (-) 1005 WP_109969651.1 type II secretion system F family protein Machinery gene
  ACKUB0_RS03845 (ACKUB0_03845) epdJ 776236..777171 (-) 936 WP_109969649.1 type II secretion system F family protein Machinery gene
  ACKUB0_RS03850 (ACKUB0_03850) epdL 777158..779056 (-) 1899 WP_109969648.1 type II/IV secretion system ATPase subunit Machinery gene
  ACKUB0_RS03855 (ACKUB0_03855) - 779217..779834 (+) 618 WP_109969647.1 hypothetical protein -
  ACKUB0_RS03860 (ACKUB0_03860) - 779866..780222 (-) 357 WP_109969646.1 hypothetical protein -
  ACKUB0_RS03865 (ACKUB0_03865) - 780226..780780 (-) 555 WP_109969645.1 aldolase -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37242.04 Da        Isoelectric Point: 9.7448

>NTDB_id=1079655 ACKUB0_RS03840 WP_109969651.1 775229..776233(-) (epdK) [Methanospirillum lacunae strain Ki8-1]
MRLFDRFKKKDEVILSPDDEAAKKILDKIELHRQYSKGNLRIIRHPFRTLTEKPVNILFASVPLGIIVFIIGFSMAIQKD
GIISLLKNSFIDDVIVITLLIILIPLVVLDLRESRRIRSIEIALPNFFRDLAGMHESGMTLQGAVHLVSMSEYGALTPHI
KQMDQRISWNFPFIDAIRAMGEEIPIPLVRRSVDLIARASEAGGDIVEVLRAAAKDSFEYVTQNTDRRNNMFIYVIIVLA
SFGVFLFVMLILVSSFLKTMADLGTTTGASSAPGFNLGGFDLALYIRIFSHGAMFQGFFSGLVAGVMGEGRVTAGLKYSV
IMLLIAWIVFRVAV

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=1079655 ACKUB0_RS03840 WP_109969651.1 775229..776233(-) (epdK) [Methanospirillum lacunae strain Ki8-1]
ATGAGACTCTTTGATCGGTTCAAAAAGAAGGATGAAGTAATACTCAGCCCTGATGATGAGGCGGCAAAGAAGATCCTTGA
TAAGATAGAACTCCACAGGCAGTATTCCAAAGGGAACCTCCGGATTATCAGACATCCCTTTCGGACCCTTACCGAAAAAC
CTGTTAATATCCTTTTTGCATCTGTCCCCCTTGGTATTATCGTCTTTATCATCGGGTTTTCAATGGCAATCCAGAAAGAT
GGGATTATATCGCTGCTCAAGAATTCTTTTATTGACGATGTCATTGTGATTACACTGCTGATCATTCTCATACCGCTGGT
TGTTCTGGATCTACGAGAATCTCGGAGAATCAGAAGTATCGAGATTGCACTCCCGAACTTCTTCCGGGATCTTGCAGGAA
TGCATGAGAGTGGAATGACTCTTCAGGGTGCAGTCCACCTTGTTTCAATGAGTGAGTATGGCGCCCTCACTCCTCATATC
AAACAGATGGATCAACGAATATCCTGGAATTTCCCTTTTATTGATGCAATTCGTGCGATGGGAGAAGAGATCCCTATCCC
ACTTGTCCGCAGAAGTGTGGATCTCATTGCCAGGGCGAGTGAAGCCGGTGGTGATATTGTTGAGGTGCTTAGAGCTGCAG
CAAAGGACTCGTTTGAGTATGTAACACAAAATACAGACCGGCGAAATAACATGTTCATCTATGTGATCATCGTTCTTGCT
TCGTTCGGTGTGTTCCTTTTTGTGATGCTCATTCTCGTTTCATCATTTTTGAAGACCATGGCGGATCTTGGGACGACAAC
CGGTGCTTCATCAGCACCCGGATTTAATCTCGGTGGTTTTGATCTCGCTCTCTATATTAGGATATTTTCACACGGTGCCA
TGTTCCAGGGTTTTTTTTCAGGGTTGGTTGCTGGTGTAATGGGTGAAGGACGGGTCACAGCGGGTCTCAAGTATTCAGTT
ATAATGCTCCTCATTGCCTGGATTGTCTTCAGGGTTGCTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(127-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V2N4X5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdK Methanoculleus thermophilus strain DSM 2373

54.397

91.916

0.5

Multiple sequence alignment