Detailed information NTG1079074

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJJTF_RS12485
• Genome accession: NZ_CP175908
• Coordinates: 2560950..2561564 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SJ22
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2555950..2566564

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJJTF_RS12455 (ACJJTF_12455)	rpsT	2556253..2556519 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACJJTF_RS12460 (ACJJTF_12460)	holA	2556536..2557577 (-)	1042	Protein_2407	DNA polymerase III subunit delta	-
ACJJTF_RS12465 (ACJJTF_12465)	-	2557617..2557768 (-)	152	Protein_2408	hypothetical protein	-
ACJJTF_RS12470 (ACJJTF_12470)	-	2557809..2557943 (+)	135	WP_003152870.1	YqzM family protein	-
ACJJTF_RS12475 (ACJJTF_12475)	comEC	2557962..2560313 (-)	2352	WP_061890729.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACJJTF_RS12480 (ACJJTF_12480)	-	2560314..2560883 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACJJTF_RS12485 (ACJJTF_12485)	comEA	2560950..2561564 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJJTF_RS12490 (ACJJTF_12490)	comER	2561623..2562444 (+)	822	WP_039254409.1	late competence protein ComER	-
ACJJTF_RS12495 (ACJJTF_12495)	-	2562513..2563250 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACJJTF_RS12500 (ACJJTF_12500)	rsfS	2563247..2563603 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACJJTF_RS12505 (ACJJTF_12505)	yqeK	2563621..2564181 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJJTF_RS12510 (ACJJTF_12510)	-	2564171..2564740 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACJJTF_RS12515 (ACJJTF_12515)	yhbY	2564751..2565041 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACJJTF_RS12520 (ACJJTF_12520)	aroE	2565035..2565877 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=1079074 ACJJTF_RS12485 WP_015240245.1 2560950..2561564(-) (comEA) [Bacillus velezensis strain SJ22]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1079074 ACJJTF_RS12485 WP_015240245.1 2560950..2561564(-) (comEA) [Bacillus velezensis strain SJ22]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363