Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACJ9PY_RS17455 Genome accession   NZ_CP175708
Coordinates   3681081..3682166 (-) Length   361 a.a.
NCBI ID   WP_372464768.1    Uniprot ID   -
Organism   Peribacillus sp. S4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3676081..3687166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ9PY_RS17415 (ACJ9PY_17415) - 3676950..3677747 (+) 798 WP_048688306.1 YqhG family protein -
  ACJ9PY_RS17420 (ACJ9PY_17420) - 3677791..3677976 (-) 186 WP_057278811.1 YqzE family protein -
  ACJ9PY_RS17425 (ACJ9PY_17425) comGG 3678092..3678478 (-) 387 WP_053535692.1 competence type IV pilus minor pilin ComGG -
  ACJ9PY_RS17430 (ACJ9PY_17430) comGF 3678471..3678950 (-) 480 WP_082854575.1 competence type IV pilus minor pilin ComGF -
  ACJ9PY_RS17435 (ACJ9PY_17435) - 3678898..3679236 (-) 339 WP_063575683.1 type II secretion system protein -
  ACJ9PY_RS17440 (ACJ9PY_17440) comGD 3679223..3679666 (-) 444 WP_034308382.1 competence type IV pilus minor pilin ComGD -
  ACJ9PY_RS17445 (ACJ9PY_17445) comGC 3679674..3679985 (-) 312 WP_034308384.1 competence type IV pilus major pilin ComGC -
  ACJ9PY_RS17450 (ACJ9PY_17450) comGB 3680063..3681097 (-) 1035 WP_063575682.1 competence type IV pilus assembly protein ComGB -
  ACJ9PY_RS17455 (ACJ9PY_17455) comGA 3681081..3682166 (-) 1086 WP_372464768.1 competence type IV pilus ATPase ComGA Machinery gene
  ACJ9PY_RS17460 (ACJ9PY_17460) - 3682261..3682641 (-) 381 WP_095678573.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACJ9PY_RS17465 (ACJ9PY_17465) - 3682857..3683558 (+) 702 WP_411738635.1 helix-turn-helix transcriptional regulator -
  ACJ9PY_RS17470 (ACJ9PY_17470) - 3683644..3683886 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  ACJ9PY_RS17475 (ACJ9PY_17475) - 3683939..3685039 (-) 1101 WP_411738636.1 class I SAM-dependent methyltransferase -
  ACJ9PY_RS17480 (ACJ9PY_17480) - 3685325..3685951 (-) 627 WP_057278817.1 MBL fold metallo-hydrolase -
  ACJ9PY_RS17485 (ACJ9PY_17485) - 3686185..3686358 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40187.85 Da        Isoelectric Point: 7.8716

>NTDB_id=1078755 ACJ9PY_RS17455 WP_372464768.1 3681081..3682166(-) (comGA) [Peribacillus sp. S4]
MNELISIEKTAEKILTRAIVESASDIHIFFRGEGPHIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSG
AITINLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSL
LHHAHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILT
TMHTRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMG
DEKEMATVSYRQLKDEIGKAVAMGYVGSQEYERLVYHETKK

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=1078755 ACJ9PY_RS17455 WP_372464768.1 3681081..3682166(-) (comGA) [Peribacillus sp. S4]
ATGAATGAATTGATATCGATTGAAAAGACTGCAGAAAAAATACTAACCCGTGCCATCGTGGAATCGGCATCGGATATCCA
CATTTTTTTTCGCGGAGAGGGGCCTCACATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTCT
TTGAAGCTGAAAGGCTGATCGCCCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGT
GCCATCACCATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGT
CATCCGCTTAATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTG
CACTCTTGAAGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACACTATATTCCCTG
CTTCACCACGCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATT
GCAAGTCCAAATCAATGAAAAGGCGGGCATTACGTATTCAGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGA
TCATGGTTGGGGAAGTCCGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACG
ACCATGCATACGAGGGACGCCCAGGGCGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGAC
TTTGATTGGCGTGACAGCGCAGCGGCTGGTTGAATTGCGATGTCTTCCGTGTAAAGGGGACTGTGCTTTAGCATGCAAAA
TGACTGCAAGGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGCCTGGCTGAAGTTCTCCGGATAATGGGA
GATGAAAAAGAAATGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGG
ATCTCAGGAATATGAACGGCTGGTCTACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.932

98.061

0.548
  pilB Haemophilus influenzae 86-028NP

37.644

96.399

0.363
  pilB Haemophilus influenzae Rd KW20

38.806

92.798

0.36

Multiple sequence alignment