Detailed information NTG1078156

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACKFA7_RS12070
• Genome accession: NZ_CP175553
• Coordinates: 2544455..2545069 (-)
• Length: 204 a.a.
• NCBI ID: WP_038459268.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain 4-9-2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2539455..2550069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKFA7_RS12040 (ACKFA7_12040)	rpsT	2539758..2540024 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACKFA7_RS12045 (ACKFA7_12045)	holA	2540041..2541082 (-)	1042	Protein_2332	DNA polymerase III subunit delta	-
ACKFA7_RS12050 (ACKFA7_12050)	-	2541122..2541273 (-)	152	Protein_2333	hypothetical protein	-
ACKFA7_RS12055 (ACKFA7_12055)	-	2541314..2541448 (+)	135	WP_003152870.1	YqzM family protein	-
ACKFA7_RS12060 (ACKFA7_12060)	comEC	2541467..2543818 (-)	2352	WP_038459264.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACKFA7_RS12065 (ACKFA7_12065)	-	2543819..2544388 (-)	570	WP_038459266.1	ComE operon protein 2	-
ACKFA7_RS12070 (ACKFA7_12070)	comEA	2544455..2545069 (-)	615	WP_038459268.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACKFA7_RS12075 (ACKFA7_12075)	comER	2545128..2545949 (+)	822	WP_012118027.1	late competence protein ComER	-
ACKFA7_RS12080 (ACKFA7_12080)	-	2546018..2546755 (-)	738	WP_038459270.1	class I SAM-dependent methyltransferase	-
ACKFA7_RS12085 (ACKFA7_12085)	rsfS	2546752..2547108 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACKFA7_RS12090 (ACKFA7_12090)	yqeK	2547112..2547687 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACKFA7_RS12095 (ACKFA7_12095)	-	2547677..2548246 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACKFA7_RS12100 (ACKFA7_12100)	yhbY	2548257..2548547 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACKFA7_RS12105 (ACKFA7_12105)	aroE	2548541..2549383 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22206.44 Da
• Isoelectric Point: 6.4939

>NTDB_id=1078156 ACKFA7_RS12070 WP_038459268.1 2544455..2545069(-) (comEA) [Bacillus amyloliquefaciens strain 4-9-2]
MTKAMMTEPLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1078156 ACKFA7_RS12070 WP_038459268.1 2544455..2545069(-) (comEA) [Bacillus amyloliquefaciens strain 4-9-2]
ATGACGAAAGCGATGATGACGGAGCCGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363