Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   ACKFA7_RS12060 Genome accession   NZ_CP175553
Coordinates   2541467..2543818 (-) Length   783 a.a.
NCBI ID   WP_038459264.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain 4-9-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2536467..2548818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKFA7_RS12025 (ACKFA7_12025) - 2536833..2537171 (-) 339 WP_038459259.1 YqxA family protein -
  ACKFA7_RS12030 (ACKFA7_12030) spoIIP 2537188..2538381 (-) 1194 WP_007612673.1 stage II sporulation protein P -
  ACKFA7_RS12035 (ACKFA7_12035) gpr 2538449..2539555 (-) 1107 WP_038459261.1 GPR endopeptidase -
  ACKFA7_RS12040 (ACKFA7_12040) rpsT 2539758..2540024 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACKFA7_RS12045 (ACKFA7_12045) holA 2540041..2541082 (-) 1042 Protein_2332 DNA polymerase III subunit delta -
  ACKFA7_RS12050 (ACKFA7_12050) - 2541122..2541273 (-) 152 Protein_2333 hypothetical protein -
  ACKFA7_RS12055 (ACKFA7_12055) - 2541314..2541448 (+) 135 WP_003152870.1 YqzM family protein -
  ACKFA7_RS12060 (ACKFA7_12060) comEC 2541467..2543818 (-) 2352 WP_038459264.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACKFA7_RS12065 (ACKFA7_12065) - 2543819..2544388 (-) 570 WP_038459266.1 ComE operon protein 2 -
  ACKFA7_RS12070 (ACKFA7_12070) comEA 2544455..2545069 (-) 615 WP_038459268.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACKFA7_RS12075 (ACKFA7_12075) comER 2545128..2545949 (+) 822 WP_012118027.1 late competence protein ComER -
  ACKFA7_RS12080 (ACKFA7_12080) - 2546018..2546755 (-) 738 WP_038459270.1 class I SAM-dependent methyltransferase -
  ACKFA7_RS12085 (ACKFA7_12085) rsfS 2546752..2547108 (-) 357 WP_007408260.1 ribosome silencing factor -
  ACKFA7_RS12090 (ACKFA7_12090) yqeK 2547112..2547687 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACKFA7_RS12095 (ACKFA7_12095) - 2547677..2548246 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  ACKFA7_RS12100 (ACKFA7_12100) yhbY 2548257..2548547 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86584.42 Da        Isoelectric Point: 9.0950

>NTDB_id=1078154 ACKFA7_RS12060 WP_038459264.1 2541467..2543818(-) (comEC) [Bacillus amyloliquefaciens strain 4-9-2]
MKYKYLLLPLAAVSATAGIAAAHVSWVLLLFLLYLLFIIVKTKQYAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKISAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTGEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=1078154 ACKFA7_RS12060 WP_038459264.1 2541467..2543818(-) (comEC) [Bacillus amyloliquefaciens strain 4-9-2]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTCCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGTATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAAGTTGACGGAGCGAAAATATCAGCTGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCACTTGAGGAAAAGAGACTTATTGAACAGCTCGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACGTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTAGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACTGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCTACGGGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(26-171)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Competence protein (Competence)

(208-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.865

98.595

0.561

Multiple sequence alignment