Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACI5EZ_RS16865 Genome accession   NZ_CP173428
Coordinates   3500957..3501697 (-) Length   246 a.a.
NCBI ID   WP_000076097.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CRAB_1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3495957..3506697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI5EZ_RS16845 aroB 3496499..3497581 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  ACI5EZ_RS16850 aroK 3497610..3498179 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ACI5EZ_RS16855 pilQ 3498191..3500356 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ACI5EZ_RS16860 pilP 3500419..3500946 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ACI5EZ_RS16865 pilO 3500957..3501697 (-) 741 WP_000076097.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACI5EZ_RS16870 pilN 3501694..3502335 (-) 642 WP_031958555.1 PilN domain-containing protein Machinery gene
  ACI5EZ_RS16875 pilM 3502335..3503393 (-) 1059 WP_005119265.1 pilus assembly protein PilM Machinery gene
  ACI5EZ_RS16880 ponA 3503554..3506109 (+) 2556 WP_000736683.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27785.55 Da        Isoelectric Point: 4.8357

>NTDB_id=1072318 ACI5EZ_RS16865 WP_000076097.1 3500957..3501697(-) (pilO) [Acinetobacter baumannii strain CRAB_1]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGFFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTIHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1072318 ACI5EZ_RS16865 WP_000076097.1 3500957..3501697(-) (pilO) [Acinetobacter baumannii strain CRAB_1]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTTTTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATTCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.187

100

0.992
  comO Acinetobacter baylyi ADP1

69.008

98.374

0.679

Multiple sequence alignment