Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACI3L5_RS15510 Genome accession   NZ_CP173389
Coordinates   3397326..3397901 (-) Length   191 a.a.
NCBI ID   WP_005432710.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3392326..3402901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L5_RS15485 (ACI3L5_15485) aroB 3392619..3393719 (-) 1101 WP_045380637.1 3-dehydroquinate synthase -
  ACI3L5_RS15490 (ACI3L5_15490) aroK 3393751..3394269 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACI3L5_RS15495 (ACI3L5_15495) pilQ 3394458..3396200 (-) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  ACI3L5_RS15500 (ACI3L5_15500) pilP 3396235..3396750 (-) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  ACI3L5_RS15505 (ACI3L5_15505) pilO 3396740..3397333 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACI3L5_RS15510 (ACI3L5_15510) pilN 3397326..3397901 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  ACI3L5_RS15515 (ACI3L5_15515) pilM 3397885..3398901 (-) 1017 WP_404973299.1 type IV pilus biogenesis protein PilM Machinery gene
  ACI3L5_RS15520 (ACI3L5_15520) - 3399080..3401647 (+) 2568 WP_404973300.1 penicillin-binding protein 1A -
  ACI3L5_RS15525 (ACI3L5_15525) oxyR 3401725..3402633 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22194.53 Da        Isoelectric Point: 9.4267

>NTDB_id=1071986 ACI3L5_RS15510 WP_005432710.1 3397326..3397901(-) (pilN) [Vibrio campbellii strain LB10]
MLYHINLLAWRENQREEHRRRFIGLILLGAIAALGVQWGIGKYYQYQQDQQQQRLDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKTVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTSRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASIKGGKQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=1071986 ACI3L5_RS15510 WP_005432710.1 3397326..3397901(-) (pilN) [Vibrio campbellii strain LB10]
ATGCTGTATCACATTAACCTTCTTGCCTGGCGTGAAAACCAACGTGAAGAGCATCGTCGTCGTTTTATTGGCTTGATTTT
ACTCGGGGCGATCGCTGCTCTGGGCGTGCAATGGGGGATCGGTAAGTACTACCAATACCAACAAGATCAGCAGCAACAGC
GCTTGGATTATCTTGCTCACTATATCTCTCAACTCGATAAACGTATTGAAGCCATGAAGATAGCAGAGCAAGAGCACAGC
AAAATTCTAGAGCGCTTAAAGACCGTTGAGGGACTGCAAAATGGGCGCAATAAAACCACTGAGTTTATGAACCTGATGCC
CTCAGTGATCCCTGAAGGCGTCTATGTCGATAAGATCAAAATGAACGACTACGAGATTGAGATCTCGGGTATTTCTGATA
GCACCTCTCGTCTTGCCACCATGCTCGATAATATGGAGCGTTCTGCCAAGCTTCTGGATGTTGATATGCACTCCATTGTC
CATGGTAAAGCGCGCTTTGGTAAAGAGTTCCAGACGTTCAAAGTCTCCTTTATGTTTAAAGATGCCTCTATAAAAGGAGG
TAAGCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

99.476

100

0.995
  pilN Vibrio cholerae strain A1552

60.452

92.67

0.56

Multiple sequence alignment