Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACI3L5_RS15515 Genome accession   NZ_CP173389
Coordinates   3397885..3398901 (-) Length   338 a.a.
NCBI ID   WP_404973299.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3392885..3403901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L5_RS15490 (ACI3L5_15490) aroK 3393751..3394269 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACI3L5_RS15495 (ACI3L5_15495) pilQ 3394458..3396200 (-) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  ACI3L5_RS15500 (ACI3L5_15500) pilP 3396235..3396750 (-) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  ACI3L5_RS15505 (ACI3L5_15505) pilO 3396740..3397333 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACI3L5_RS15510 (ACI3L5_15510) pilN 3397326..3397901 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  ACI3L5_RS15515 (ACI3L5_15515) pilM 3397885..3398901 (-) 1017 WP_404973299.1 type IV pilus biogenesis protein PilM Machinery gene
  ACI3L5_RS15520 (ACI3L5_15520) - 3399080..3401647 (+) 2568 WP_404973300.1 penicillin-binding protein 1A -
  ACI3L5_RS15525 (ACI3L5_15525) oxyR 3401725..3402633 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  ACI3L5_RS15530 (ACI3L5_15530) - 3402808..3403536 (+) 729 WP_005432732.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37846.27 Da        Isoelectric Point: 6.3137

>NTDB_id=1071987 ACI3L5_RS15515 WP_404973299.1 3397885..3398901(-) (pilM) [Vibrio campbellii strain LB10]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKDMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1071987 ACI3L5_RS15515 WP_404973299.1 3397885..3398901(-) (pilM) [Vibrio campbellii strain LB10]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGATATGAAAAAAGAGCTACCTTTTCGGGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCTGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTGGCAGACGACGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.929

100

0.979
  pilM Vibrio cholerae strain A1552

49.123

100

0.497

Multiple sequence alignment