Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACIO05_RS03580 Genome accession   NZ_CP173232
Coordinates   700240..700782 (+) Length   180 a.a.
NCBI ID   WP_110479719.1    Uniprot ID   A0AAE5TGK8
Organism   Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695240..705782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO05_RS03560 (ACIO05_03560) glnB 695754..696092 (+) 339 WP_017805299.1 nitrogen regulatory protein P-II -
  ACIO05_RS03565 (ACIO05_03565) - 696095..697129 (+) 1035 WP_410679128.1 AI-2E family transporter -
  ACIO05_RS03570 (ACIO05_03570) - 697159..698730 (+) 1572 WP_410679127.1 tetratricopeptide repeat protein -
  ACIO05_RS03575 (ACIO05_03575) - 699037..700206 (+) 1170 WP_410679126.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACIO05_RS03580 (ACIO05_03580) pilF/pilF2 700240..700782 (+) 543 WP_110479719.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIO05_RS03585 (ACIO05_03585) dprA 700927..702027 (+) 1101 WP_410679125.1 DNA-processing protein DprA Machinery gene
  ACIO05_RS03590 (ACIO05_03590) rsmD 702024..702632 (-) 609 WP_264245187.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACIO05_RS03595 (ACIO05_03595) ftsY 702788..704281 (+) 1494 WP_410681550.1 signal recognition particle-docking protein FtsY -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20727.78 Da        Isoelectric Point: 8.0752

>NTDB_id=1071014 ACIO05_RS03580 WP_110479719.1 700240..700782(+) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-3]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENIRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=1071014 ACIO05_RS03580 WP_110479719.1 700240..700782(+) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-3]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAGCTTGGTATTGGCTATCTTGAGCAAAATGAACTTGGGTTGG
CGAAGCAAAATTTAGATAAAGCCTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCGTTGGCATATTTTTATCAG
CTTCAAGGGGAGAATATTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCAGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.153

98.333

0.483
  pilF Glaesserella parasuis strain SC1401

43.407

100

0.439

Multiple sequence alignment