Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACIO03_RS04925 Genome accession   NZ_CP173231
Coordinates   1062807..1063349 (-) Length   180 a.a.
NCBI ID   WP_110479719.1    Uniprot ID   A0AAE5TGK8
Organism   Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1057807..1068349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO03_RS04910 (ACIO03_04910) ftsY 1059308..1060801 (-) 1494 WP_410681550.1 signal recognition particle-docking protein FtsY -
  ACIO03_RS04915 (ACIO03_04915) rsmD 1060957..1061565 (+) 609 WP_264245187.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACIO03_RS04920 (ACIO03_04920) dprA 1061562..1062662 (-) 1101 WP_410679125.1 DNA-processing protein DprA Machinery gene
  ACIO03_RS04925 (ACIO03_04925) pilF/pilF2 1062807..1063349 (-) 543 WP_110479719.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIO03_RS04930 (ACIO03_04930) - 1063383..1064552 (-) 1170 WP_410679126.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACIO03_RS04935 (ACIO03_04935) - 1064859..1066430 (-) 1572 WP_410681551.1 tetratricopeptide repeat protein -
  ACIO03_RS04940 (ACIO03_04940) - 1066460..1067494 (-) 1035 WP_410679128.1 AI-2E family transporter -
  ACIO03_RS04945 (ACIO03_04945) glnB 1067497..1067835 (-) 339 WP_017805299.1 nitrogen regulatory protein P-II -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20727.78 Da        Isoelectric Point: 8.0752

>NTDB_id=1070985 ACIO03_RS04925 WP_110479719.1 1062807..1063349(-) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-4]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENIRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=1070985 ACIO03_RS04925 WP_110479719.1 1062807..1063349(-) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-4]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAGCTTGGTATTGGCTATCTTGAGCAAAATGAACTTGGGTTGG
CGAAGCAAAATTTAGATAAAGCCTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCGTTGGCATATTTTTATCAG
CTTCAAGGGGAGAATATTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCAGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.153

98.333

0.483
  pilF Glaesserella parasuis strain SC1401

43.407

100

0.439

Multiple sequence alignment