Detailed information NTG1069427

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: ACFELE_RS25150
• Genome accession: NZ_CP172856
• Coordinates: 5363115..5364452 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain 238632E_ID011
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5358115..5369452

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELE_RS25125 (ACFELE_25125)	pgeF	5358258..5358986 (-)	729	WP_003102588.1	peptidoglycan editing factor PgeF	-
ACFELE_RS25130 (ACFELE_25130)	rluD	5358983..5359945 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
ACFELE_RS25135 (ACFELE_25135)	-	5360091..5361116 (+)	1026	WP_003102590.1	outer membrane protein assembly factor BamD	-
ACFELE_RS25140 (ACFELE_25140)	-	5361273..5361518 (+)	246	WP_003102591.1	PP0621 family protein	-
ACFELE_RS25145 (ACFELE_25145)	pilS	5361508..5363100 (+)	1593	WP_003123394.1	two-component system sensor histidine kinase PilS	Regulator
ACFELE_RS25150 (ACFELE_25150)	pilR	5363115..5364452 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
ACFELE_RS25155 (ACFELE_25155)	thiO	5364498..5365592 (-)	1095	WP_003119424.1	glycine oxidase ThiO	-
ACFELE_RS25160 (ACFELE_25160)	-	5365727..5366236 (+)	510	WP_003102595.1	Tfp pilus assembly protein FimT/FimU	-
ACFELE_RS25165 (ACFELE_25165)	-	5366342..5366848 (+)	507	WP_003102598.1	Tfp pilus assembly protein FimT/FimU	-
ACFELE_RS25170 (ACFELE_25170)	pilV	5366839..5367396 (+)	558	WP_003119423.1	type 4a pilus minor pilin PilV	Machinery gene
ACFELE_RS25175 (ACFELE_25175)	pilW	5367393..5368217 (+)	825	WP_003119422.1	type 4a pilus minor pilin PilW	-
ACFELE_RS25180 (ACFELE_25180)	pilX	5368214..5368801 (+)	588	WP_003112826.1	type 4a pilus minor pilin PilX	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=1069427 ACFELE_RS25150 WP_003094694.1 5363115..5364452(+) (pilR) [Pseudomonas aeruginosa strain 238632E_ID011]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=1069427 ACFELE_RS25150 WP_003094694.1 5363115..5364452(+) (pilR) [Pseudomonas aeruginosa strain 238632E_ID011]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384