Detailed information NTG1069217

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: ACFELB_RS25525
• Genome accession: NZ_CP172850
• Coordinates: 5465543..5466880 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain 238638E_ID017
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5460543..5471880

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELB_RS25500 (ACFELB_25500)	pgeF	5460686..5461414 (-)	729	WP_031693031.1	peptidoglycan editing factor PgeF	-
ACFELB_RS25505 (ACFELB_25505)	rluD	5461411..5462373 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
ACFELB_RS25510 (ACFELB_25510)	-	5462519..5463544 (+)	1026	WP_003102590.1	outer membrane protein assembly factor BamD	-
ACFELB_RS25515 (ACFELB_25515)	-	5463701..5463946 (+)	246	WP_009316269.1	PP0621 family protein	-
ACFELB_RS25520 (ACFELB_25520)	pilS	5463936..5465528 (+)	1593	WP_003123394.1	two-component system sensor histidine kinase PilS	Regulator
ACFELB_RS25525 (ACFELB_25525)	pilR	5465543..5466880 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
ACFELB_RS25530 (ACFELB_25530)	thiO	5466926..5468020 (-)	1095	WP_023084775.1	glycine oxidase ThiO	-
ACFELB_RS25535 (ACFELB_25535)	-	5468155..5468664 (+)	510	WP_196691309.1	GspH/FimT family pseudopilin	-
ACFELB_RS25540 (ACFELB_25540)	-	5468770..5469276 (+)	507	WP_003102598.1	Tfp pilus assembly protein FimT/FimU	-
ACFELB_RS25545 (ACFELB_25545)	pilV	5469267..5469824 (+)	558	WP_003119423.1	type 4a pilus minor pilin PilV	Machinery gene
ACFELB_RS25550 (ACFELB_25550)	pilW	5469821..5470645 (+)	825	WP_003119422.1	type 4a pilus minor pilin PilW	-
ACFELB_RS25555 (ACFELB_25555)	pilX	5470642..5471229 (+)	588	WP_003112826.1	type 4a pilus minor pilin PilX	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=1069217 ACFELB_RS25525 WP_003094694.1 5465543..5466880(+) (pilR) [Pseudomonas aeruginosa strain 238638E_ID017]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=1069217 ACFELB_RS25525 WP_003094694.1 5465543..5466880(+) (pilR) [Pseudomonas aeruginosa strain 238638E_ID017]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACTCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384