Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACFMPA_RS12965 Genome accession   NZ_CP172605
Coordinates   2530840..2531457 (-) Length   205 a.a.
NCBI ID   WP_032722140.1    Uniprot ID   -
Organism   Bacillus sp. SG20032     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2525840..2536457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFMPA_RS12935 (ACFMPA_12935) rpsT 2526142..2526408 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  ACFMPA_RS12940 (ACFMPA_12940) holA 2526423..2527466 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  ACFMPA_RS12945 (ACFMPA_12945) - 2527506..2527655 (-) 150 WP_003229985.1 hypothetical protein -
  ACFMPA_RS12950 (ACFMPA_12950) - 2527696..2527830 (+) 135 WP_003229983.1 YqzM family protein -
  ACFMPA_RS12955 (ACFMPA_12955) comEC 2527870..2530200 (-) 2331 WP_326225400.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACFMPA_RS12960 (ACFMPA_12960) - 2530204..2530773 (-) 570 WP_003229978.1 ComE operon protein 2 -
  ACFMPA_RS12965 (ACFMPA_12965) comEA 2530840..2531457 (-) 618 WP_032722140.1 competence protein ComEA Machinery gene
  ACFMPA_RS12970 (ACFMPA_12970) comER 2531541..2532362 (+) 822 WP_015714289.1 late competence protein ComER -
  ACFMPA_RS12975 (ACFMPA_12975) - 2532428..2533171 (-) 744 WP_069322765.1 class I SAM-dependent methyltransferase -
  ACFMPA_RS12980 (ACFMPA_12980) rsfS 2533168..2533524 (-) 357 WP_041333759.1 ribosome silencing factor -
  ACFMPA_RS12985 (ACFMPA_12985) yqeK 2533542..2534102 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACFMPA_RS12990 (ACFMPA_12990) - 2534092..2534661 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ACFMPA_RS12995 (ACFMPA_12995) yhbY 2534673..2534963 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACFMPA_RS13000 (ACFMPA_13000) aroE 2534957..2535799 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21748.46 Da        Isoelectric Point: 4.8354

>NTDB_id=1068236 ACFMPA_RS12965 WP_032722140.1 2530840..2531457(-) (comEA) [Bacillus sp. SG20032]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=1068236 ACFMPA_RS12965 WP_032722140.1 2530840..2531457(-) (comEA) [Bacillus sp. SG20032]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment