Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACHGMI_RS14690 Genome accession   NZ_CP172417
Coordinates   2685298..2685915 (+) Length   205 a.a.
NCBI ID   WP_041344905.1    Uniprot ID   -
Organism   Bacillus subtilis strain AKPS2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2680298..2690915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHGMI_RS14655 aroE 2680956..2681798 (+) 843 WP_017696285.1 shikimate dehydrogenase -
  ACHGMI_RS14660 yhbY 2681792..2682082 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACHGMI_RS14665 nadD 2682094..2682663 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ACHGMI_RS14670 yqeK 2682653..2683213 (+) 561 WP_017696284.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACHGMI_RS14675 rsfS 2683231..2683587 (+) 357 WP_014480315.1 ribosome silencing factor -
  ACHGMI_RS14680 yqeM 2683584..2684327 (+) 744 WP_017696283.1 class I SAM-dependent DNA methyltransferase -
  ACHGMI_RS14685 comER 2684393..2685214 (-) 822 WP_004398597.1 late competence protein ComER -
  ACHGMI_RS14690 comEA 2685298..2685915 (+) 618 WP_041344905.1 competence protein ComEA Machinery gene
  ACHGMI_RS14695 comEB 2685982..2686551 (+) 570 WP_003229978.1 ComE operon protein 2 -
  ACHGMI_RS14700 comEC 2686555..2688885 (+) 2331 WP_017696281.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACHGMI_RS14705 yqzM 2688925..2689059 (-) 135 WP_003229983.1 YqzM family protein -
  ACHGMI_RS14710 - 2689100..2689249 (+) 150 WP_003229985.1 hypothetical protein -
  ACHGMI_RS14715 holA 2689289..2690332 (+) 1044 WP_017696280.1 DNA polymerase III subunit delta -
  ACHGMI_RS14720 rpsT 2690347..2690613 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21762.55 Da        Isoelectric Point: 4.9552

>NTDB_id=1067598 ACHGMI_RS14690 WP_041344905.1 2685298..2685915(+) (comEA) [Bacillus subtilis strain AKPS2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEGNNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAKAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=1067598 ACHGMI_RS14690 WP_041344905.1 2685298..2685915(+) (comEA) [Bacillus subtilis strain AKPS2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGAAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGGAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGACCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTAAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.818

100

0.395

Multiple sequence alignment