Detailed information NTG1067375

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACHZEV_RS13490
• Genome accession: NZ_CP172401
• Coordinates: 2761178..2761675 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain M22.2
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2756178..2766675

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACHZEV_RS13450 (ACHZEV_13450)	-	2756222..2757208 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACHZEV_RS13455 (ACHZEV_13455)	-	2757208..2757537 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACHZEV_RS13460 (ACHZEV_13460)	-	2757534..2758157 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACHZEV_RS13465 (ACHZEV_13465)	comGA	2758209..2759183 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACHZEV_RS13470 (ACHZEV_13470)	comGB	2759155..2760225 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACHZEV_RS13475 (ACHZEV_13475)	comGC	2760239..2760550 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACHZEV_RS13480 (ACHZEV_13480)	comGD	2760528..2760974 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACHZEV_RS13485 (ACHZEV_13485)	comGE	2760961..2761260 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACHZEV_RS13490 (ACHZEV_13490)	comGF	2761178..2761675 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACHZEV_RS13495 (ACHZEV_13495)	-	2761772..2761918 (+)	147	WP_001789879.1	hypothetical protein	-
ACHZEV_RS13500 (ACHZEV_13500)	-	2761908..2762432 (+)	525	WP_001015120.1	shikimate kinase	-
ACHZEV_RS13505 (ACHZEV_13505)	gcvT	2762591..2763682 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACHZEV_RS13510 (ACHZEV_13510)	gcvPA	2763702..2765048 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACHZEV_RS13515 (ACHZEV_13515)	gcvPB	2765041..2766513 (+)	1473	WP_316133277.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1067375 ACHZEV_RS13490 WP_001825340.1 2761178..2761675(+) (comGF) [Staphylococcus aureus strain M22.2]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1067375 ACHZEV_RS13490 WP_001825340.1 2761178..2761675(+) (comGF) [Staphylococcus aureus strain M22.2]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97