Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   ACF0H7_RS03015 Genome accession   NZ_CP172305
Coordinates   617055..618644 (-) Length   529 a.a.
NCBI ID   WP_397451334.1    Uniprot ID   -
Organism   Pseudomonas sp. NA-150     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 612055..623644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0H7_RS02990 (ACF0H7_02990) - 612188..612997 (-) 810 WP_397451329.1 PilW family protein -
  ACF0H7_RS02995 (ACF0H7_02995) pilV 612994..613527 (-) 534 WP_397451330.1 type IV pilus modification protein PilV Machinery gene
  ACF0H7_RS03000 (ACF0H7_03000) - 613524..614078 (-) 555 WP_397451331.1 GspH/FimT family pseudopilin -
  ACF0H7_RS03005 (ACF0H7_03005) thiO 614323..615426 (+) 1104 WP_397451332.1 glycine oxidase ThiO -
  ACF0H7_RS03010 (ACF0H7_03010) pilR 615712..617052 (-) 1341 WP_397451333.1 sigma-54-dependent transcriptional regulator Regulator
  ACF0H7_RS03015 (ACF0H7_03015) pilS 617055..618644 (-) 1590 WP_397451334.1 sensor histidine kinase Regulator
  ACF0H7_RS03020 (ACF0H7_03020) - 618634..618873 (-) 240 WP_397451335.1 PP0621 family protein -
  ACF0H7_RS03025 (ACF0H7_03025) - 618992..620011 (-) 1020 WP_397451336.1 outer membrane protein assembly factor BamD -
  ACF0H7_RS03030 (ACF0H7_03030) rluD 620161..621123 (+) 963 WP_397451337.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACF0H7_RS03035 (ACF0H7_03035) pgeF 621132..621869 (+) 738 WP_397453812.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58382.45 Da        Isoelectric Point: 6.7796

>NTDB_id=1066129 ACF0H7_RS03015 WP_397451334.1 617055..618644(-) (pilS) [Pseudomonas sp. NA-150]
MTADRLSLSSDQAQRILRLYHLYRLAIGVTLVLLISSNLDGELLELVNARLFRAGCWVYLIVNIMVGVLLNKPEHSTQIF
SLALADVVMLSALFYAGGGPPSGIGNLLIASVAISNVLLRGRSGLMIAAVAASGIIYLTFYLSFDRAPISNYYMQAGSLG
TLSFAAALLVQALSRRMRLSETLAEERAADVDNLEELNALILQRMRTGILVLDAKRRVLLANKGALNLLGQDHLIGEIDG
YSPQLVERLRQWLLNPMLRSHSVVTAAGGPELQPSFIALNRYGQRQTLIFLEDLSEISQQAQQLKLAALGRLTAGIAHEI
RNPLGAISHAAQLLKESEELSPPDQRLSQIIHDQTRRMDLVIENVLQLSRRRQAEPQLLDLKGWLEQFVSEVRGAATANQ
RLHVDIGTGTLTTRMDPEQLTQVMTNLLQNGLRYSGKFHDVAQVWLTLLHEPISDLPIIEVMDDGPGVATEQLSKVFEPF
FTTESKGTGLGLYISRELCESNQAHLDYRPRDGGGSCFRITFAHPLKLS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1066129 ACF0H7_RS03015 WP_397451334.1 617055..618644(-) (pilS) [Pseudomonas sp. NA-150]
GTGACCGCTGATCGACTGTCGTTGAGCAGCGATCAGGCGCAACGCATTCTTCGGCTTTACCACCTTTATCGCCTGGCCAT
CGGCGTCACCCTGGTCCTGCTGATCTCCAGCAATCTGGACGGTGAGTTGTTGGAGCTGGTCAACGCCAGGCTGTTTCGCG
CCGGCTGCTGGGTGTACCTGATCGTCAACATCATGGTCGGCGTGCTGCTGAACAAGCCGGAGCACTCGACGCAGATTTTC
AGCCTGGCCCTGGCCGACGTCGTGATGCTCTCGGCACTGTTCTACGCCGGCGGCGGCCCGCCCAGTGGCATCGGCAACCT
GCTGATCGCTTCTGTGGCCATCAGTAACGTGTTGCTGCGCGGCCGTAGTGGCCTGATGATCGCCGCCGTCGCGGCCAGCG
GCATTATTTACCTGACGTTTTACCTCAGCTTCGATCGAGCACCGATCTCCAACTACTACATGCAAGCCGGTTCGCTGGGC
ACCCTGAGTTTCGCGGCCGCCCTGCTGGTACAGGCACTCAGCCGACGCATGCGCCTCAGTGAAACACTGGCAGAAGAGCG
TGCAGCAGACGTCGACAACCTGGAAGAGCTCAACGCGCTGATCCTGCAACGGATGCGCACCGGCATTCTGGTGCTGGACG
CCAAGCGCCGGGTGTTGCTGGCCAATAAGGGCGCTTTAAACCTACTGGGACAGGACCATTTAATCGGTGAAATCGACGGC
TATTCCCCTCAACTGGTGGAGCGTCTGCGCCAATGGCTACTCAACCCGATGTTGCGCTCGCACAGCGTGGTGACCGCCGC
CGGTGGACCGGAACTGCAACCCAGCTTCATTGCCCTGAACCGCTATGGCCAACGTCAGACTCTGATATTTCTCGAAGACC
TGTCGGAAATCTCCCAACAAGCGCAGCAACTCAAGCTCGCCGCGCTGGGGCGCCTGACCGCCGGGATCGCCCACGAAATT
CGCAATCCACTGGGCGCCATCAGCCATGCAGCCCAACTGCTCAAAGAATCCGAAGAACTGAGCCCGCCGGATCAACGCCT
GAGCCAAATCATTCATGATCAAACCCGGCGCATGGATCTGGTGATCGAAAACGTCTTGCAACTGTCTCGAAGACGTCAGG
CCGAACCGCAGCTATTGGATCTAAAGGGCTGGCTTGAGCAGTTCGTCAGCGAGGTACGCGGCGCGGCGACAGCCAATCAA
CGACTGCACGTAGACATCGGCACCGGCACCTTGACCACGCGCATGGACCCGGAGCAATTGACCCAGGTGATGACCAACCT
GCTGCAGAATGGCTTGCGCTATAGCGGCAAATTCCACGATGTCGCTCAAGTTTGGCTAACATTACTGCACGAGCCGATCA
GTGACCTGCCGATCATCGAAGTGATGGATGACGGTCCTGGGGTAGCCACCGAGCAGCTGAGCAAGGTATTCGAACCCTTC
TTCACTACGGAAAGCAAAGGTACCGGTCTGGGCCTCTATATTTCGCGCGAGCTGTGCGAAAGCAATCAGGCACATCTGGA
TTACAGACCCCGAGACGGCGGCGGTAGTTGTTTCCGCATTACGTTCGCCCATCCGCTGAAATTGAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(308-372)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(416-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

63.962

100

0.641

Multiple sequence alignment