Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACHKST_RS12415 Genome accession   NZ_CP172011
Coordinates   2536978..2537592 (-) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain Bv.g1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531978..2542592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHKST_RS12385 rpsT 2532278..2532544 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACHKST_RS12390 holA 2532561..2533602 (-) 1042 Protein_2393 DNA polymerase III subunit delta -
  ACHKST_RS12395 - 2533642..2533796 (-) 155 Protein_2394 hypothetical protein -
  ACHKST_RS12400 - 2533837..2533971 (+) 135 WP_003152870.1 YqzM family protein -
  ACHKST_RS12405 comEC 2533990..2536341 (-) 2352 WP_071391596.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACHKST_RS12410 - 2536342..2536911 (-) 570 WP_003152868.1 ComE operon protein 2 -
  ACHKST_RS12415 comEA 2536978..2537592 (-) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACHKST_RS12420 comER 2537651..2538472 (+) 822 WP_101562284.1 late competence protein ComER -
  ACHKST_RS12425 - 2538541..2539278 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  ACHKST_RS12430 rsfS 2539275..2539631 (-) 357 WP_003152864.1 ribosome silencing factor -
  ACHKST_RS12435 yqeK 2539649..2540209 (-) 561 WP_031378934.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACHKST_RS12440 - 2540199..2540768 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  ACHKST_RS12445 yhbY 2540779..2541069 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  ACHKST_RS12450 aroE 2541063..2541905 (-) 843 WP_031378933.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=1064846 ACHKST_RS12415 WP_013352942.1 2536978..2537592(-) (comEA) [Bacillus velezensis strain Bv.g1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1064846 ACHKST_RS12415 WP_013352942.1 2536978..2537592(-) (comEA) [Bacillus velezensis strain Bv.g1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCCTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment