Detailed information NTG1064405

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PN836_RS08030
• Genome accession: NZ_CP171821
• Coordinates: 1831372..1832250 (+)
• Length: 292 a.a.
• NCBI ID: WP_395344724.1
• Uniprot ID: -
• Organism: Ningiella sp. W23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1826372..1837250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PN836_RS08005 (PN836_008135)	-	1826416..1827186 (-)	771	WP_395344719.1	GntR family transcriptional regulator	-
PN836_RS08010 (PN836_008140)	ampE	1827338..1828255 (-)	918	WP_395344720.1	regulatory signaling modulator protein AmpE	-
PN836_RS08015 (PN836_008145)	nadC	1828429..1829310 (+)	882	WP_395344721.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PN836_RS08020 (PN836_008150)	-	1829567..1830013 (+)	447	WP_395344722.1	pilin	-
PN836_RS08025 (PN836_008155)	pilC	1830090..1831301 (+)	1212	WP_395344723.1	type II secretion system F family protein	Machinery gene
PN836_RS08030 (PN836_008160)	pilD	1831372..1832250 (+)	879	WP_395344724.1	prepilin peptidase	Machinery gene
PN836_RS08035 (PN836_008165)	-	1832496..1833902 (+)	1407	WP_395344725.1	DUF945 family protein	-
PN836_RS08040 (PN836_008170)	coaE	1834029..1834655 (+)	627	WP_395344726.1	dephospho-CoA kinase	-
PN836_RS08045 (PN836_008175)	zapD	1834692..1835534 (+)	843	WP_395344727.1	cell division protein ZapD	-
PN836_RS08050 (PN836_008180)	yacG	1835593..1835829 (+)	237	WP_395344728.1	DNA gyrase inhibitor YacG	-
PN836_RS08055 (PN836_008185)	-	1835826..1836728 (+)	903	WP_395344729.1	aspartoacylase	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 31973.79 Da
• Isoelectric Point: 5.2277

>NTDB_id=1064405 PN836_RS08030 WP_395344724.1 1831372..1832250(+) (pilD) [Ningiella sp. W23]
MIDLLINSPAYFIGFTFIISLMIGSFLNVVIYRLPIMMKRSWEAEIEAYQHPEEAQSHGAGGESDTFNLVKPDSTCPKCG
HKIRAWENIPVISWLALRAKCSSCKTPISARYPAIELLTAVLSALAAWQLGFSPITLAFILGTWMLVAMTFIDIDEMLLP
DSLTLSLLWLGLLVTAIYNTAGLQDAVFGAIAGYMSLFTVYWAFKLLTGKEGMGFGDFKLLAAIGVWVGWQHLPIVILLS
SVVGAIVGITLMFIQKKDAQLAIPFGPYLAAAGYLTVLYGDTIADWYLSTLG

Nucleotide

• Length: 879 bp

>NTDB_id=1064405 PN836_RS08030 WP_395344724.1 1831372..1832250(+) (pilD) [Ningiella sp. W23]
ATGATTGATTTGCTAATAAACTCGCCCGCCTATTTTATAGGCTTTACCTTTATCATCAGTTTGATGATTGGAAGCTTTTT
GAATGTTGTCATCTATCGACTGCCGATAATGATGAAGCGCTCGTGGGAGGCTGAAATTGAAGCCTATCAACATCCTGAAG
AGGCACAATCTCACGGTGCAGGTGGCGAATCAGACACCTTTAATCTGGTAAAACCGGACTCTACTTGCCCTAAGTGCGGT
CACAAAATTCGTGCGTGGGAAAACATTCCTGTTATCAGTTGGCTAGCGCTTCGAGCTAAATGCAGTTCATGCAAAACGCC
TATCTCCGCTCGCTATCCAGCTATCGAACTTCTTACCGCTGTGCTTAGCGCGCTTGCTGCATGGCAGCTTGGATTCTCTC
CCATAACTTTGGCATTCATATTGGGCACTTGGATGCTGGTTGCGATGACCTTTATCGATATTGACGAGATGCTTTTGCCC
GATTCCCTCACCCTGTCATTGTTATGGCTGGGGCTATTAGTCACCGCTATATACAATACAGCAGGCTTACAAGATGCTGT
GTTTGGCGCCATTGCAGGATACATGTCGCTGTTTACTGTTTATTGGGCCTTTAAACTACTCACCGGAAAAGAAGGCATGG
GCTTTGGTGACTTTAAGCTGCTCGCTGCAATAGGCGTGTGGGTAGGTTGGCAACACCTACCCATCGTTATTTTACTTTCA
TCGGTTGTTGGCGCCATAGTTGGCATCACGCTTATGTTCATTCAGAAAAAAGACGCTCAACTCGCTATTCCATTTGGTCC
CTATCTCGCGGCTGCAGGTTATTTGACGGTGCTCTATGGAGACACTATCGCCGATTGGTATTTATCTACGCTTGGGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (141-249)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.724	99.315	0.514
pilD	Vibrio campbellii strain DS40M4	52.465	97.26	0.51
pilD	Neisseria gonorrhoeae MS11	46.918	100	0.469
pilD	Acinetobacter baumannii D1279779	47.232	92.808	0.438
pilD	Acinetobacter nosocomialis M2	46.863	92.808	0.435