Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACHD8M_RS13065 Genome accession   NZ_CP171807
Coordinates   2616089..2616703 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain BRI3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2611089..2621703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHD8M_RS13035 rpsT 2611392..2611658 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACHD8M_RS13040 holA 2611675..2612716 (-) 1042 Protein_2522 DNA polymerase III subunit delta -
  ACHD8M_RS13045 - 2612751..2612907 (-) 157 Protein_2523 hypothetical protein -
  ACHD8M_RS13050 - 2612948..2613082 (+) 135 WP_003152870.1 YqzM family protein -
  ACHD8M_RS13055 comEC 2613101..2615452 (-) 2352 WP_159354394.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACHD8M_RS13060 - 2615453..2616022 (-) 570 WP_003152868.1 ComE operon protein 2 -
  ACHD8M_RS13065 comEA 2616089..2616703 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACHD8M_RS13070 comER 2616762..2617583 (+) 822 WP_159354395.1 late competence protein ComER -
  ACHD8M_RS13075 - 2617652..2618389 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  ACHD8M_RS13080 rsfS 2618386..2618742 (-) 357 WP_007408260.1 ribosome silencing factor -
  ACHD8M_RS13085 yqeK 2618760..2619320 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACHD8M_RS13090 - 2619310..2619879 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  ACHD8M_RS13095 yhbY 2619890..2620180 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  ACHD8M_RS13100 aroE 2620174..2621016 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=1064352 ACHD8M_RS13065 WP_015240245.1 2616089..2616703(-) (comEA) [Bacillus velezensis strain BRI3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1064352 ACHD8M_RS13065 WP_015240245.1 2616089..2616703(-) (comEA) [Bacillus velezensis strain BRI3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment