Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   AAA996_RS10645 Genome accession   NZ_AP028606
Coordinates   2071537..2072835 (-) Length   432 a.a.
NCBI ID   WP_000867610.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain Sep1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2066537..2077835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA996_RS10625 (TKY121773_20540) rplI 2067692..2068144 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  AAA996_RS10630 (TKY121773_20550) - 2068141..2070114 (-) 1974 WP_000715129.1 DHH family phosphoesterase -
  AAA996_RS10635 (TKY121773_20560) raiA 2070250..2070798 (-) 549 WP_000599107.1 ribosome-associated translation inhibitor RaiA -
  AAA996_RS10640 (TKY121773_20570) comFC/cflB 2070878..2071540 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  AAA996_RS10645 (TKY121773_20580) comFA/cflA 2071537..2072835 (-) 1299 WP_000867610.1 DEAD/DEAH box helicase Machinery gene
  AAA996_RS10650 (TKY121773_20590) - 2072891..2073526 (+) 636 WP_000395739.1 YigZ family protein -
  AAA996_RS10655 (TKY121773_20600) cysK 2073811..2074731 (+) 921 WP_000029862.1 cysteine synthase A -
  AAA996_RS10660 - 2074970..2075335 (-) 366 Protein_2061 IS66 family transposase -
  AAA996_RS10665 - 2075317..2075480 (+) 164 Protein_2062 transposase -
  AAA996_RS10670 (TKY121773_20620) tnpB 2075594..2075848 (-) 255 WP_078373100.1 IS66 family insertion sequence element accessory protein TnpB -
  AAA996_RS10675 (TKY121773_20630) tsf 2076164..2077204 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49577.51 Da        Isoelectric Point: 8.4382

>NTDB_id=106244 AAA996_RS10645 WP_000867610.1 2071537..2072835(-) (comFA/cflA) [Streptococcus pneumoniae strain Sep1]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=106244 AAA996_RS10645 WP_000867610.1 2071537..2072835(-) (comFA/cflA) [Streptococcus pneumoniae strain Sep1]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAGAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGTATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCGGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAATAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae D39

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae R6

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae TIGR4

98.843

100

0.988
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment