Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   ACF0HT_RS00185 Genome accession   NZ_CP171612
Coordinates   43618..44967 (-) Length   449 a.a.
NCBI ID   WP_394523706.1    Uniprot ID   -
Organism   Staphylococcus xylosus strain 14BME18     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 38618..49967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0HT_RS00155 (ACF0HT_00155) - 38994..39461 (-) 468 WP_232136788.1 hypothetical protein -
  ACF0HT_RS00160 (ACF0HT_00160) - 39481..40134 (-) 654 WP_071331190.1 hypothetical protein -
  ACF0HT_RS00165 (ACF0HT_00165) - 40229..40735 (-) 507 WP_394523705.1 DUF1643 domain-containing protein -
  ACF0HT_RS00170 (ACF0HT_00170) - 40749..41060 (-) 312 WP_011302036.1 DUF960 family protein -
  ACF0HT_RS00175 (ACF0HT_00175) - 41156..41494 (-) 339 WP_011302037.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ACF0HT_RS00180 (ACF0HT_00180) ccrB 41968..43596 (-) 1629 WP_071331188.1 cassette chromosome recombinase CcrB Machinery gene
  ACF0HT_RS00185 (ACF0HT_00185) ccrA 43618..44967 (-) 1350 WP_394523706.1 cassette chromosome recombinase CcrA Machinery gene
  ACF0HT_RS00190 (ACF0HT_00190) cch1 45503..47290 (-) 1788 WP_071331186.1 cassette chromosome replicative helicase -
  ACF0HT_RS00195 (ACF0HT_00195) ssb 47290..47535 (-) 246 WP_071331185.1 cassette chromosome ssDNA-binding protein -
  ACF0HT_RS00200 (ACF0HT_00200) - 47673..48716 (+) 1044 WP_071331184.1 ACP synthase -
  ACF0HT_RS00205 (ACF0HT_00205) - 48787..49152 (+) 366 Protein_38 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52310.10 Da        Isoelectric Point: 10.1532

>NTDB_id=1062324 ACF0HT_RS00185 WP_394523706.1 43618..44967(-) (ccrA) [Staphylococcus xylosus strain 14BME18]
MKQAMGYLRQSTTKQQSLPAQKQAIKTLAEKHNIQHITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSFHDGYFDMDKAFDRLKLNIFISVAELESDNIGEQVSNGIKEKARQGKLITTHAPFGYHYK
RGTFTINKNESPTVIAVFNYYLQGNGYKKITQFLEEDNKLLNRKPYQVRNIIMNPNYCGRVINKYGQYDNMFPSIVSTNM
YEQAQALRSQKQIKRTPSDNQLKQKIKCPYCGSTLTNMTIRKKNHALRYYVCSRNMNASHFVCEFKGINAPKLETQVLTT
CQDFFQNQQLYLKINDVIQKRIKKQRALETRSTLTQEQLIDKLAKGAIDAETFRKQSQSLLQQSKPTLSINEQQIQRSFE
NVIQQRFTLNMLYPYIDEIHISKNKSLVGIYFKNEPLNIVNQATQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1062324 ACF0HT_RS00185 WP_394523706.1 43618..44967(-) (ccrA) [Staphylococcus xylosus strain 14BME18]
ATGAAACAAGCAATGGGTTACTTACGTCAAAGTACAACAAAACAACAATCACTTCCGGCACAGAAACAAGCCATCAAGAC
ATTAGCTGAAAAACACAATATTCAACACATCACATTTTATAGCGATAAGCAATCAGGACGCACTGATAAGCGAAACGGTT
ACCAGCAAATTACTGAACTGATTCAACAAGGACAATGTGACGTACTATGTTGCTATCGTCTGAATCGACTTCATCGTAAT
CTAAAAAATGCATTAAAACTCATGAAATTGTGCCAAAAATATCATGTTCATATCTTAAGTTTTCATGATGGCTATTTTGA
TATGGATAAAGCATTCGATCGGCTTAAACTTAATATTTTCATCAGCGTGGCCGAATTAGAATCTGATAATATTGGCGAAC
AAGTAAGCAACGGGATAAAAGAAAAAGCGCGTCAGGGTAAATTGATAACTACACACGCCCCCTTTGGTTATCACTACAAA
CGTGGTACATTCACGATAAATAAGAATGAATCACCAACGGTAATTGCCGTATTCAATTACTATCTTCAAGGTAATGGCTA
TAAAAAAATTACACAGTTCTTAGAAGAAGATAACAAACTACTCAACCGGAAACCTTATCAAGTACGTAATATTATTATGA
ATCCCAATTACTGTGGTCGTGTTATCAATAAATATGGACAATATGACAACATGTTTCCGTCTATTGTTTCTACAAATATG
TACGAACAAGCGCAAGCTCTACGATCGCAAAAACAAATCAAACGAACACCTTCAGACAATCAACTCAAACAAAAAATCAA
ATGCCCTTATTGCGGGTCAACCCTTACCAATATGACAATTAGAAAAAAGAACCATGCATTACGTTACTATGTCTGTTCTC
GAAATATGAATGCATCTCATTTTGTCTGTGAATTCAAAGGGATTAATGCGCCAAAACTAGAAACTCAAGTCTTAACTACA
TGTCAGGACTTCTTTCAAAATCAACAACTTTATTTAAAAATCAACGATGTAATTCAAAAACGGATCAAAAAACAAAGGGC
CCTAGAAACTAGGAGCACACTTACACAAGAACAATTGATAGATAAATTAGCTAAAGGAGCCATCGATGCAGAAACGTTCA
GAAAACAATCTCAATCGTTACTTCAACAATCAAAACCAACACTATCAATAAATGAGCAACAAATTCAAAGATCATTTGAA
AATGTAATTCAACAACGCTTCACGTTAAACATGTTATACCCATATATTGATGAAATTCATATTTCTAAAAACAAAAGCCT
TGTGGGAATCTATTTCAAAAATGAACCGCTAAACATTGTAAACCAAGCTACGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

80.401

100

0.804
  ccrA Staphylococcus aureus N315

75.724

100

0.757

Multiple sequence alignment