Detailed information NTG0106080

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: AAA980_RS10425
• Genome accession: NZ_AP028604
• Coordinates: 1978730..1979191 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250540.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain Pne3
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1973730..1984191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAA980_RS10390 (TKY182805_20280)	-	1974034..1974858 (-)	825	WP_001832473.1	membrane protein insertase YidC	-
AAA980_RS10395 (TKY182805_20290)	rnpA	1974833..1975204 (-)	372	WP_000739244.1	ribonuclease P protein component	-
AAA980_RS10400	-	1975221..1975352 (-)	132	WP_000768904.1	hypothetical protein	-
AAA980_RS10405 (TKY182805_20300)	-	1975353..1976543 (-)	1191	WP_000167752.1	acetate kinase	-
AAA980_RS10410 (TKY182805_20310)	comYH	1976594..1977547 (-)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
AAA980_RS10415	-	1977608..1978202 (-)	595	Protein_2027	methyltransferase domain-containing protein	-
AAA980_RS10420 (TKY182805_20340)	comGG/cglG	1978339..1978752 (-)	414	WP_000265620.1	competence type IV pilus minor pilin ComGG	Machinery gene
AAA980_RS10425 (TKY182805_20350)	comGF/cglF	1978730..1979191 (-)	462	WP_000250540.1	competence type IV pilus minor pilin ComGF	Machinery gene
AAA980_RS10430 (TKY182805_20360)	comGE/cglE	1979154..1979456 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
AAA980_RS10435 (TKY182805_20370)	comGD/cglD	1979419..1979823 (-)	405	WP_000588023.1	competence type IV pilus minor pilin ComGD	Machinery gene
AAA980_RS10440 (TKY182805_20380)	comGC/cglC	1979816..1980142 (-)	327	WP_050260822.1	comG operon protein ComGC	Machinery gene
AAA980_RS10445 (TKY182805_20390)	comGB/cglB	1980144..1981160 (-)	1017	WP_073177425.1	competence type IV pilus assembly protein ComGB	Machinery gene
AAA980_RS10450 (TKY182805_20400)	comGA/cglA/cilD	1981108..1982049 (-)	942	WP_000249559.1	competence type IV pilus ATPase ComGA	Machinery gene
AAA980_RS10455 (TKY182805_20410)	-	1982125..1982490 (-)	366	WP_000286415.1	DUF1033 family protein	-
AAA980_RS10460 (TKY182805_20420)	-	1982757..1984012 (+)	1256	WP_088804233.1	ISL3 family transposase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17850.40 Da
• Isoelectric Point: 8.0016

>NTDB_id=106080 AAA980_RS10425 WP_000250540.1 1978730..1979191(-) (comGF/cglF) [Streptococcus pneumoniae strain Pne3]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=106080 AAA980_RS10425 WP_000250540.1 1978730..1979191(-) (comGF/cglF) [Streptococcus pneumoniae strain Pne3]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	92.157	100	0.922