Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   ACF0HW_RS06700 Genome accession   NZ_CP171208
Coordinates   1297339..1297776 (+) Length   145 a.a.
NCBI ID   WP_012117983.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain HN11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1292339..1302776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0HW_RS06675 - 1292348..1293649 (-) 1302 WP_012117986.1 hemolysin family protein -
  ACF0HW_RS06680 - 1293795..1294745 (+) 951 WP_007408319.1 magnesium transporter CorA family protein -
  ACF0HW_RS06685 comGA 1294942..1296012 (+) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  ACF0HW_RS06690 comGB 1295999..1297036 (+) 1038 WP_012117984.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACF0HW_RS06695 comGC 1297083..1297349 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  ACF0HW_RS06700 comGD 1297339..1297776 (+) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACF0HW_RS06705 comGE 1297760..1298074 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ACF0HW_RS06710 comGF 1297983..1298483 (+) 501 WP_012117981.1 competence type IV pilus minor pilin ComGF -
  ACF0HW_RS06715 comGG 1298484..1298861 (+) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACF0HW_RS06720 - 1298918..1299097 (+) 180 WP_003153093.1 YqzE family protein -
  ACF0HW_RS06725 - 1299137..1299466 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  ACF0HW_RS06730 tapA 1299725..1300396 (+) 672 WP_012117978.1 amyloid fiber anchoring/assembly protein TapA -
  ACF0HW_RS06735 sipW 1300368..1300952 (+) 585 WP_012117977.1 signal peptidase I SipW -
  ACF0HW_RS06740 tasA 1301017..1301802 (+) 786 WP_007408329.1 biofilm matrix protein TasA -
  ACF0HW_RS06745 sinR 1301850..1302185 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ACF0HW_RS06750 sinI 1302219..1302392 (-) 174 WP_003153105.1 anti-repressor SinI Regulator

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16286.74 Da        Isoelectric Point: 10.2475

>NTDB_id=1060476 ACF0HW_RS06700 WP_012117983.1 1297339..1297776(+) (comGD) [Bacillus amyloliquefaciens strain HN11]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPAYTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1060476 ACF0HW_RS06700 WP_012117983.1 1297339..1297776(+) (comGD) [Bacillus amyloliquefaciens strain HN11]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGCCTACACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGACTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATCACGGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

56.849

100

0.572

Multiple sequence alignment