Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACFFRD_RS20910 Genome accession   NZ_CP171148
Coordinates   3589175..3591181 (+) Length   668 a.a.
NCBI ID   WP_392477152.1    Uniprot ID   -
Organism   Nostoc sp. C110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3584175..3596181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFFRD_RS20895 dnaK 3585782..3587740 (-) 1959 WP_114080202.1 molecular chaperone DnaK -
  ACFFRD_RS20900 grpE 3587967..3588716 (-) 750 WP_114080203.1 nucleotide exchange factor GrpE -
  ACFFRD_RS20905 - 3588853..3588984 (+) 132 WP_392477150.1 hypothetical protein -
  ACFFRD_RS20910 pilB/pilB1 3589175..3591181 (+) 2007 WP_392477152.1 GspE/PulE family protein Machinery gene
  ACFFRD_RS20915 pilT 3591296..3592423 (+) 1128 WP_392477153.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFFRD_RS20920 - 3592528..3593751 (+) 1224 WP_179071231.1 type II secretion system F family protein -
  ACFFRD_RS20925 - 3593880..3594440 (+) 561 WP_114080207.1 hypothetical protein -
  ACFFRD_RS20930 - 3594567..3595571 (-) 1005 WP_114080208.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74569.39 Da        Isoelectric Point: 5.1746

>NTDB_id=1060301 ACFFRD_RS20910 WP_392477152.1 3589175..3591181(+) (pilB/pilB1) [Nostoc sp. C110]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSIGNTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVRQKHLEQEKFTDINQDLENLGNLDLADAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLKEAFDPLPKKIIPAVTARFKIIANLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASSDVAITFYKANSLTLDASAEAKTKNQLCSKCNG
VGYKGRCGVYEVMRVTETLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=1060301 ACFFRD_RS20910 WP_392477152.1 3589175..3591181(+) (pilB/pilB1) [Nostoc sp. C110]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
GCAATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCCGACCCTTAACGGAAG
TACTAGAGTCAATTACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGTGTTGAATTCCTCGATCCGGAAGTCAACTCCATTGGCAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTGGATATCTGCCGTCGCCATCGTTTAGTACCACTATCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTCGCTCCAGATAATCTAGAAGCTTCCGATGACCTGAACCGCATCTTGCGCCCCCAAGGACTGGCATTACAG
CGCATGGTGATTACACAGGAAGACTACCAGCAGCTAATCAACCAATATCTGGATGAAATGGCTGTTCGGCAAAAGCACCT
CGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTCGGAAATCTTGATCTTGCAGATGCTCCTGAAGAAA
TGGAGGCTGATTTAGGAGCAGCGATGAAGGGTGCGGAGGATGCCCCAGTAATCAACCTAGTCAACAGAATCCTTGCTAAA
GCCTTACATGAGGGCGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATCCGCTTTCGGAAGGATGGGGT
ACTGAAGGAAGCTTTCGATCCCCTACCGAAAAAAATCATCCCGGCGGTGACAGCCCGATTTAAAATCATCGCCAATCTAG
ACATTGCTGAACGCCGTCTACCCCAAGATGGACGTATCCGCCGGATGTTTGAGGGACGTAAGGTGGACTTCCGGGTGAAT
ACCTTGCCCAGCCGCTACGGGGAAAAGGTGGTACTGCGGATTTTGGATAACTCCTCCACCCAACTGGGATTAGATAAGTT
AATTACCGATCCAGAGACTTTACATATTGTCCAGGATATGGTTAGCCGTCCCTTTGGGCTAATTTTGGTAACTGGGCCAA
CTGGTTCTGGGAAAACAACCTCGTTGTATTCTGCACTATCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTAGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTAGATTTTGCTACTGCTCT
GCGGGCTTTCTTGCGACAAGATCCAGATGTGCTACTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCTATTG
AAGCTGCCTTGACTGGTCACTTAGTATTAACTACTTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAA
ATGGGGATTGAGCCTTTCATGGTTTCTAGTTCGCTAATTGGCGTTCTAGCTCAACGTTTGGTGCGGCGTGTATGTTCTGA
ATGTCGTATTCCCTACACTCCTACAACCGAGGAACTGGCTCGTTATGGTCTATCAGCTTCTTCTGATGTCGCAATCACCT
TCTATAAGGCTAACAGTTTGACATTAGATGCGAGCGCCGAAGCCAAAACCAAAAATCAGCTTTGCTCAAAATGTAATGGT
GTTGGCTACAAAGGTCGTTGTGGTGTTTATGAAGTCATGCGAGTCACTGAAACTCTGCAAACTCTGATCAACGAAGATGC
ACCCACGGAACGCATCAAGGAAGTGGCAATAGAAGAGGGCATGAAAACCTTGCTGGCTTATAGTTTGGACTTAGTGCGTG
AGGGTTCCACCACTCTAGAAGAAGTAGAACGAGTGACGTTTACTGATACTGGTTTGGAAGCAGAGTTAAAAGCCAAACGC
AAGACTGGTCTTACCTGCCGCAGTTGTGATGCCACATTGAAACAAGAATGGCTGGATTGTCCCTACTGTATGACATCTCG
GTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.518

100

0.669

Multiple sequence alignment