Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACFG4S_RS10565 Genome accession   NZ_CP170991
Coordinates   2307825..2309831 (-) Length   668 a.a.
NCBI ID   WP_392534749.1    Uniprot ID   -
Organism   Nostoc sp. C117     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2302825..2314831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFG4S_RS10545 - 2302973..2304136 (-) 1164 WP_392534745.1 DUF58 domain-containing protein -
  ACFG4S_RS10550 - 2304240..2304797 (-) 558 WP_392534746.1 hypothetical protein -
  ACFG4S_RS10555 - 2305135..2306358 (-) 1224 WP_392534747.1 type II secretion system F family protein -
  ACFG4S_RS10560 pilT 2306597..2307724 (-) 1128 WP_392534748.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFG4S_RS10565 pilB/pilB1 2307825..2309831 (-) 2007 WP_392534749.1 GspE/PulE family protein Machinery gene
  ACFG4S_RS10570 grpE 2310370..2311107 (+) 738 WP_392534750.1 nucleotide exchange factor GrpE -
  ACFG4S_RS10575 dnaK 2311430..2313388 (+) 1959 WP_392535956.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74561.45 Da        Isoelectric Point: 5.2482

>NTDB_id=1059945 ACFG4S_RS10565 WP_392534749.1 2307825..2309831(-) (pilB/pilB1) [Nostoc sp. C117]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNSEQMRQALIESRKSGRPLTEVLESLTGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSVGNTMVGNLIDTLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDDLAVRQKHLEQEKFTDINQDLENLGNLDIEDAPDDMEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIIASLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLRIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGLLASSEVEVTFYKANSLTLEAIAEAKTKNHLCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAIEEGMKTLLAYSIDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCDATLKPEMLDCPYCLTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=1059945 ACFG4S_RS10565 WP_392534749.1 2307825..2309831(-) (pilB/pilB1) [Nostoc sp. C117]
ATGACTTACTCGTCTCCACAACGGCGCAGTACCGCTTTAACTACCAGAACAGAATTCTCGCCCTTTGGCAACAAGCTAGT
CCAATCTGGCTATGTCAATAGCGAACAGATGAGGCAAGCACTAATTGAAAGCCGCAAGTCTGGTAGACCTTTAACGGAAG
TACTAGAGTCCCTTACTGGACAACAGCTATCACCCGAGTTACTCAGGCAATATAAAAAACAGCAGCTATTTGAACTTAAA
ATACTTTACGGCGTAGAATTCCTCGATCCGGAAGTCAACTCCGTTGGTAACACAATGGTGGGGAACCTGATTGATACCCT
GATCCCAGTGGATATTTGCCGTCGTCATCGTTTAGTACCACTGTCAAAACACGAAGACCAAACCCCGCCTTCAGTTTTAG
TGGCGATGGTTGCTCCAGATAATTTAGAGGCTTCCGATGACCTGAATCGCATCTTGCGCCCCCAAGGCTTGGCATTGCAG
CGCATGGTGATTACACAGGAGGACTACCAACAGCTAATCAACCAATACTTGGATGACTTGGCTGTTCGGCAAAAGCACCT
AGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAATCTCGGAAATCTCGACATTGAAGATGCCCCTGATGATA
TGGAGGCTGATCTTGGGGCAGCTATGAAGGGTGCTGAGGATGCCCCAGTTATCAACTTAGTTAACAGAATCTTGGCAAAA
GCTTTGCATGAGAAGGTTTCTGATATTCACATCGAACCTCAGGAAGAAAACTTACGCATTCGCTTTCGGAAGGATGGGGT
ACTACGTGAAGCTTTTGATCCCCTACCGAAAAAAATCATCCCAGCGGTGACAGCTCGGTTTAAAATCATCGCCAGTCTAG
ACATTGCTGAACGCCGTCTACCCCAGGATGGACGCATCCGGCGGCTGTTTGAGGGACGTAAGGTGGACTTCCGTGTTAAT
ACCTTACCCAGTCGCTATGGGGAAAAGGTGGTGCTACGGATTTTGGATAATTCTTCCACCCAACTCGGATTGAATAAGTT
AATTACCGATCCAGAGACTTTGCGTATTGTCCAGGATATGGTCAGCCGTCCCTTTGGTCTGATTTTGGTAACAGGGCCAA
CTGGTTCCGGGAAAACAACGTCGCTGTATTCTGCACTATCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTAGAA
GACCCAATTGAATACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAGAAAGGGCTGGATTTTGCGACAGCATT
GCGGGCTTTCTTGCGACAAGATCCAGATGTGCTACTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACCGCGATTG
AGGCTGCCTTGACTGGTCACTTAGTATTAACTACTTTACATACCAATGATGCTCCAGGAGCGATCGCTCGTTTAGGAGAA
ATGGGAATTGAGCCTTTCATGGTTTCTAGTTCTTTAATTGGCGTTTTGGCTCAACGTCTGGTGCGGCGGGTATGTTCTGA
ATGTCGCATTGCTTACACTCCCACAACCGAGGAACTGGCTCGTTATGGTCTATTAGCTTCCTCTGAGGTAGAAGTCACCT
TCTACAAAGCTAACAGTTTGACATTAGAAGCGATCGCAGAAGCCAAAACCAAAAATCATCTTTGCCCAGCCTGTAACGGC
GTCGGCTACAAAGGGCGTTGTGGTGTCTATGAAGTCATGCGAGTCACCGAAAACCTCCAAACCCTGATCAACGAAGAAGC
ACCTACAGAACGCATCAAGGAAGTCGCAATAGAAGAGGGTATGAAAACTTTGCTGGCTTACAGCATAGACTTAGTGCGCC
AAGGTTCTACCACCCTCGAAGAAGTAGAACGGGTGACGTTTACTGATACTGGTTTGGAAGCCGAGTTAAAAGCCAAACGC
AAAACAGGTCTTACCTGTCGGACTTGTGATGCCACATTGAAACCAGAAATGCTGGATTGTCCGTACTGTCTGACATCTCG
GTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.179

100

0.656
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.725

96.257

0.373
  pilB Vibrio campbellii strain DS40M4

39.573

91.168

0.361

Multiple sequence alignment