Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   AABA08_RS10330 Genome accession   NZ_AP028602
Coordinates   2019195..2020493 (-) Length   432 a.a.
NCBI ID   WP_000867588.1    Uniprot ID   A0A6I3U5D2
Organism   Streptococcus pneumoniae strain PF2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2014195..2025493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABA08_RS10310 (TKY092604_19940) rplI 2015350..2015802 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  AABA08_RS10315 (TKY092604_19950) - 2015799..2017772 (-) 1974 WP_000715109.1 DHH family phosphoesterase -
  AABA08_RS10320 (TKY092604_19960) hpf 2017908..2018456 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AABA08_RS10325 (TKY092604_19970) comFC/cflB 2018536..2019198 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  AABA08_RS10330 (TKY092604_19980) comFA/cflA 2019195..2020493 (-) 1299 WP_000867588.1 DEAD/DEAH box helicase Machinery gene
  AABA08_RS10335 (TKY092604_19990) - 2020549..2021184 (+) 636 WP_000395739.1 YigZ family protein -
  AABA08_RS10340 (TKY092604_20000) cysK 2021469..2022395 (+) 927 WP_000029875.1 cysteine synthase A -
  AABA08_RS10345 (TKY092604_20010) tsf 2022517..2023557 (-) 1041 WP_000808068.1 translation elongation factor Ts -
  AABA08_RS10350 (TKY092604_20020) rpsB 2023636..2024415 (-) 780 WP_000268459.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49537.46 Da        Isoelectric Point: 8.2050

>NTDB_id=105929 AABA08_RS10330 WP_000867588.1 2019195..2020493(-) (comFA/cflA) [Streptococcus pneumoniae strain PF2]
MKVNLDYLGCLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=105929 AABA08_RS10330 WP_000867588.1 2019195..2020493(-) (comFA/cflA) [Streptococcus pneumoniae strain PF2]
ATGAAAGTAAATTTAGATTATCTCGGTTGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I3U5D2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

93.056

100

0.931
  comFA/cflA Streptococcus mitis SK321

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

39.609

94.676

0.375

Multiple sequence alignment