Detailed information NTG1058961

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACFIGL_RS13855
• Genome accession: NZ_CP170734
• Coordinates: 2640208..2640825 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain M51
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2635208..2645825

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFIGL_RS13825 (ACFIGL_13835)	rpsT	2635510..2635776 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACFIGL_RS13830 (ACFIGL_13840)	holA	2635791..2636834 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
ACFIGL_RS13835 (ACFIGL_13845)	-	2636874..2637023 (-)	150	WP_003229985.1	hypothetical protein	-
ACFIGL_RS13840 (ACFIGL_13850)	yqzM	2637064..2637198 (+)	135	WP_003229983.1	YqzM family protein	-
ACFIGL_RS13845 (ACFIGL_13855)	comEC	2637238..2639568 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFIGL_RS13850 (ACFIGL_13860)	comEB	2639572..2640141 (-)	570	WP_003229978.1	ComE operon protein 2	-
ACFIGL_RS13855 (ACFIGL_13865)	comEA	2640208..2640825 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
ACFIGL_RS13860 (ACFIGL_13870)	comER	2640909..2641730 (+)	822	WP_004398597.1	late competence protein ComER	-
ACFIGL_RS13865 (ACFIGL_13875)	yqeM	2641796..2642539 (-)	744	WP_003229973.1	class I SAM-dependent DNA methyltransferase	-
ACFIGL_RS13870 (ACFIGL_13880)	rsfS	2642536..2642892 (-)	357	WP_003229971.1	ribosome silencing factor	-
ACFIGL_RS13875 (ACFIGL_13885)	yqeK	2642910..2643470 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFIGL_RS13880 (ACFIGL_13890)	nadD	2643460..2644029 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ACFIGL_RS13885 (ACFIGL_13895)	yhbY	2644041..2644331 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACFIGL_RS13890 (ACFIGL_13900)	aroE	2644325..2645167 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1058961 ACFIGL_RS13855 WP_004398514.1 2640208..2640825(-) (comEA) [Bacillus subtilis strain M51]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1058961 ACFIGL_RS13855 WP_004398514.1 2640208..2640825(-) (comEA) [Bacillus subtilis strain M51]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39