Detailed information NTG1058689

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACFXAA_RS18740
• Genome accession: NZ_CP170717
• Coordinates: 3825081..3825695 (+)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5014
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3820081..3830695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAA_RS18705 (ACFXAA_18705)	aroE	3820768..3821610 (+)	843	WP_039254404.1	shikimate dehydrogenase	-
ACFXAA_RS18710 (ACFXAA_18710)	yhbY	3821604..3821894 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACFXAA_RS18715 (ACFXAA_18715)	-	3821905..3822474 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFXAA_RS18720 (ACFXAA_18720)	yqeK	3822464..3823024 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFXAA_RS18725 (ACFXAA_18725)	rsfS	3823042..3823398 (+)	357	WP_007408260.1	ribosome silencing factor	-
ACFXAA_RS18730 (ACFXAA_18730)	-	3823395..3824132 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACFXAA_RS18735 (ACFXAA_18735)	comER	3824201..3825022 (-)	822	WP_039254409.1	late competence protein ComER	-
ACFXAA_RS18740 (ACFXAA_18740)	comEA	3825081..3825695 (+)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFXAA_RS18745 (ACFXAA_18745)	-	3825762..3826331 (+)	570	WP_003152868.1	ComE operon protein 2	-
ACFXAA_RS18750 (ACFXAA_18750)	comEC	3826332..3828683 (+)	2352	WP_317859499.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFXAA_RS18755 (ACFXAA_18755)	-	3828702..3828836 (-)	135	WP_003152870.1	YqzM family protein	-
ACFXAA_RS18760 (ACFXAA_18760)	-	3828877..3829028 (+)	152	Protein_3631	hypothetical protein	-
ACFXAA_RS18765 (ACFXAA_18765)	holA	3829068..3830109 (+)	1042	Protein_3632	DNA polymerase III subunit delta	-
ACFXAA_RS18770 (ACFXAA_18770)	rpsT	3830126..3830392 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=1058689 ACFXAA_RS18740 WP_015240245.1 3825081..3825695(+) (comEA) [Bacillus amyloliquefaciens strain JP5014]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1058689 ACFXAA_RS18740 WP_015240245.1 3825081..3825695(+) (comEA) [Bacillus amyloliquefaciens strain JP5014]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363