Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   AAA982_RS10450 Genome accession   NZ_AP028601
Coordinates   2022105..2023403 (-) Length   432 a.a.
NCBI ID   WP_000867605.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PF1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2017105..2028403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA982_RS10430 (TKY113292_20130) rplI 2018260..2018712 (-) 453 WP_338168293.1 50S ribosomal protein L9 -
  AAA982_RS10435 (TKY113292_20140) - 2018709..2020682 (-) 1974 WP_000715110.1 DHH family phosphoesterase -
  AAA982_RS10440 (TKY113292_20150) raiA 2020818..2021366 (-) 549 WP_000599104.1 ribosome-associated translation inhibitor RaiA -
  AAA982_RS10445 (TKY113292_20160) comFC/cflB 2021446..2022108 (-) 663 WP_000649974.1 ComF family protein Machinery gene
  AAA982_RS10450 (TKY113292_20170) comFA/cflA 2022105..2023403 (-) 1299 WP_000867605.1 DEAD/DEAH box helicase Machinery gene
  AAA982_RS10455 (TKY113292_20180) - 2023459..2024094 (+) 636 WP_000395739.1 YigZ family protein -
  AAA982_RS10460 (TKY113292_20190) cysK 2024379..2025299 (+) 921 WP_000029866.1 cysteine synthase A -
  AAA982_RS10465 - 2025374..2025903 (-) 530 Protein_2022 transposase -
  AAA982_RS10470 (TKY113292_20210) - 2025885..2026172 (+) 288 Protein_2023 transposase -
  AAA982_RS10475 (TKY113292_20220) tnpB 2026372..2026650 (-) 279 WP_317649194.1 IS66 family insertion sequence element accessory protein TnpB -
  AAA982_RS10480 (TKY113292_20230) tsf 2026896..2027936 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49620.59 Da        Isoelectric Point: 8.4382

>NTDB_id=105853 AAA982_RS10450 WP_000867605.1 2022105..2023403(-) (comFA/cflA) [Streptococcus pneumoniae strain PF1]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=105853 AAA982_RS10450 WP_000867605.1 2022105..2023403(-) (comFA/cflA) [Streptococcus pneumoniae strain PF1]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCAATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment