Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACFXAD_RS08710 Genome accession   NZ_CP170653
Coordinates   1693595..1694665 (+) Length   356 a.a.
NCBI ID   WP_053573196.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1688595..1699665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS08680 (ACFXAD_08680) - 1689068..1689313 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  ACFXAD_RS08685 (ACFXAD_08685) - 1689550..1689930 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACFXAD_RS08695 (ACFXAD_08695) - 1690137..1690967 (+) 831 WP_053573195.1 STAS domain-containing protein -
  ACFXAD_RS08700 (ACFXAD_08700) - 1691005..1692306 (-) 1302 WP_012117986.1 hemolysin family protein -
  ACFXAD_RS08705 (ACFXAD_08705) - 1692452..1693402 (+) 951 WP_015417820.1 magnesium transporter CorA family protein -
  ACFXAD_RS08710 (ACFXAD_08710) comGA 1693595..1694665 (+) 1071 WP_053573196.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFXAD_RS08715 (ACFXAD_08715) comGB 1694652..1695689 (+) 1038 WP_053573197.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFXAD_RS08720 (ACFXAD_08720) comGC 1695694..1696002 (+) 309 WP_079979070.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFXAD_RS08725 (ACFXAD_08725) comGD 1695992..1696429 (+) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFXAD_RS08730 (ACFXAD_08730) comGE 1696413..1696727 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ACFXAD_RS08735 (ACFXAD_08735) comGF 1696636..1697136 (+) 501 WP_256052909.1 competence type IV pilus minor pilin ComGF -
  ACFXAD_RS08740 (ACFXAD_08740) comGG 1697137..1697514 (+) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACFXAD_RS08745 (ACFXAD_08745) - 1697571..1697750 (+) 180 WP_003153093.1 YqzE family protein -
  ACFXAD_RS08750 (ACFXAD_08750) - 1697790..1698119 (-) 330 WP_039254490.1 DUF3889 domain-containing protein -
  ACFXAD_RS08755 (ACFXAD_08755) tapA 1698378..1699049 (+) 672 WP_053573199.1 amyloid fiber anchoring/assembly protein TapA -
  ACFXAD_RS08760 (ACFXAD_08760) sipW 1699021..1699605 (+) 585 WP_015240205.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40555.89 Da        Isoelectric Point: 9.4504

>NTDB_id=1058489 ACFXAD_RS08710 WP_053573196.1 1693595..1694665(+) (comGA) [Bacillus amyloliquefaciens strain JP5011]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGYALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1058489 ACFXAD_RS08710 WP_053573196.1 1693595..1694665(+) (comGA) [Bacillus amyloliquefaciens strain JP5011]
GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCTATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment