Detailed information NTG1058425

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACFW99_RS12700
• Genome accession: NZ_CP170651
• Coordinates: 2616208..2616822 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5025
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2611208..2621822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFW99_RS12670 (ACFW99_12670)	rpsT	2611511..2611777 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACFW99_RS12675 (ACFW99_12675)	holA	2611794..2612835 (-)	1042	Protein_2457	DNA polymerase III subunit delta	-
ACFW99_RS12680 (ACFW99_12680)	-	2612875..2613026 (-)	152	Protein_2458	hypothetical protein	-
ACFW99_RS12685 (ACFW99_12685)	-	2613067..2613201 (+)	135	WP_003152870.1	YqzM family protein	-
ACFW99_RS12690 (ACFW99_12690)	comEC	2613220..2615571 (-)	2352	WP_317859499.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFW99_RS12695 (ACFW99_12695)	-	2615572..2616141 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACFW99_RS12700 (ACFW99_12700)	comEA	2616208..2616822 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFW99_RS12705 (ACFW99_12705)	comER	2616881..2617702 (+)	822	WP_039254409.1	late competence protein ComER	-
ACFW99_RS12710 (ACFW99_12710)	-	2617771..2618508 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACFW99_RS12715 (ACFW99_12715)	rsfS	2618505..2618861 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACFW99_RS12720 (ACFW99_12720)	yqeK	2618879..2619439 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFW99_RS12725 (ACFW99_12725)	-	2619429..2619998 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFW99_RS12730 (ACFW99_12730)	yhbY	2620009..2620299 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACFW99_RS12735 (ACFW99_12735)	aroE	2620293..2621135 (-)	843	WP_033574150.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=1058425 ACFW99_RS12700 WP_015240245.1 2616208..2616822(-) (comEA) [Bacillus amyloliquefaciens strain JP5025]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1058425 ACFW99_RS12700 WP_015240245.1 2616208..2616822(-) (comEA) [Bacillus amyloliquefaciens strain JP5025]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363