Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   ACFMSI_RS01490 Genome accession   NZ_CP170579
Coordinates   337185..338810 (+) Length   541 a.a.
NCBI ID   WP_381543575.1    Uniprot ID   -
Organism   Staphylococcus aureus strain JB2E56     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 303602..338337 337185..338810 flank -1152


Gene organization within MGE regions


Location: 303602..338810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFMSI_RS01325 (ACFMSI_01325) - 303602..304414 (-) 813 WP_002504699.1 abortive infection system antitoxin AbiGi family protein -
  ACFMSI_RS01330 (ACFMSI_01330) - 304605..306119 (+) 1515 WP_145426217.1 class I SAM-dependent DNA methyltransferase -
  ACFMSI_RS01335 (ACFMSI_01335) - 306109..307389 (+) 1281 WP_381543572.1 restriction endonuclease subunit S -
  ACFMSI_RS01340 (ACFMSI_01340) - 307373..310492 (+) 3120 WP_381543573.1 type I restriction endonuclease subunit R -
  ACFMSI_RS01345 (ACFMSI_01345) - 310566..310787 (-) 222 WP_002504278.1 hypothetical protein -
  ACFMSI_RS01350 (ACFMSI_01350) - 310802..311311 (-) 510 WP_145426223.1 DUF1643 domain-containing protein -
  ACFMSI_RS01355 (ACFMSI_01355) - 311327..311638 (-) 312 WP_145426225.1 DUF960 family protein -
  ACFMSI_RS01360 (ACFMSI_01360) - 311732..312070 (-) 339 WP_002449468.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ACFMSI_RS01365 (ACFMSI_01365) - 312144..312491 (-) 348 Protein_272 recombinase family protein -
  ACFMSI_RS01370 (ACFMSI_01370) - 312589..313119 (-) 531 WP_145426227.1 hypothetical protein -
  ACFMSI_RS01375 (ACFMSI_01375) - 313558..313689 (-) 132 Protein_274 SAUGI family uracil-DNA glycosylase inhibitor -
  ACFMSI_RS01380 (ACFMSI_01380) ccrC 313797..315473 (-) 1677 WP_126116859.1 cassette chromosome recombinase CcrC -
  ACFMSI_RS01385 (ACFMSI_01385) - 315698..317314 (-) 1617 WP_001015575.1 DUF5906 domain-containing protein -
  ACFMSI_RS01390 (ACFMSI_01390) - 317314..317682 (-) 369 WP_001058155.1 hypothetical protein -
  ACFMSI_RS01395 (ACFMSI_01395) - 317675..318784 (-) 1110 WP_000283873.1 DNA polymerase -
  ACFMSI_RS01400 (ACFMSI_01400) - 318979..320967 (-) 1989 WP_001038847.1 hypothetical protein -
  ACFMSI_RS01405 (ACFMSI_01405) - 321129..322058 (-) 930 WP_000750311.1 YafY family protein -
  ACFMSI_RS01410 (ACFMSI_01410) - 322139..322567 (+) 429 WP_000671996.1 VOC family protein -
  ACFMSI_RS01415 (ACFMSI_01415) - 322627..323301 (-) 675 Protein_282 IS6 family transposase -
  ACFMSI_RS01420 (ACFMSI_01420) - 323342..323449 (-) 108 Protein_283 beta-lactam sensor/signal transducer MecR1 -
  ACFMSI_RS01425 (ACFMSI_01425) mecA 323546..325555 (+) 2010 WP_063852638.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  ACFMSI_RS01430 (ACFMSI_01430) - 325601..326029 (-) 429 WP_000872606.1 MaoC family dehydratase -
  ACFMSI_RS01435 (ACFMSI_01435) - 326126..326869 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  ACFMSI_RS01440 (ACFMSI_01440) - 327786..328175 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  ACFMSI_RS01445 (ACFMSI_01445) - 328211..328885 (+) 675 Protein_288 IS6 family transposase -
  ACFMSI_RS01450 (ACFMSI_01450) - 328986..329804 (-) 819 WP_002484875.1 CPBP family intramembrane glutamic endopeptidase -
  ACFMSI_RS01455 (ACFMSI_01455) - 329841..330167 (-) 327 WP_012085243.1 helix-turn-helix domain-containing protein -
  ACFMSI_RS01460 (ACFMSI_01460) - 331397..333322 (-) 1926 WP_070657144.1 heavy metal translocating P-type ATPase -
  ACFMSI_RS01465 (ACFMSI_01465) - 333625..333951 (-) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  ACFMSI_RS01470 (ACFMSI_01470) - 334158..334517 (+) 360 WP_046465687.1 hypothetical protein -
  ACFMSI_RS01475 (ACFMSI_01475) - 334629..334967 (-) 339 Protein_294 recombinase family protein -
  ACFMSI_RS01480 (ACFMSI_01480) - 334976..335614 (+) 639 Protein_295 hypothetical protein -
  ACFMSI_RS01485 (ACFMSI_01485) ccrA 335814..337163 (+) 1350 WP_381543574.1 cassette chromosome recombinase CcrA Machinery gene
  ACFMSI_RS01490 (ACFMSI_01490) ccrB 337185..338810 (+) 1626 WP_381543575.1 cassette chromosome recombinase CcrB Machinery gene

Sequence

Protein


Download         Length: 541 a.a.        Molecular weight: 62282.31 Da        Isoelectric Point: 9.7362

>NTDB_id=1058110 ACFMSI_RS01490 WP_381543575.1 337185..338810(+) (ccrB) [Staphylococcus aureus strain JB2E56]
MDKMKKKLVGGYIRVSTERQVEGYSIEGQITQIEQYCQFNSYELVDIYADRGISGKSMNRPELQRMLNDAKNGKLDCVMV
YKTNRLARNTSDLLTIVEELHRQNVEFFSLSERMEVKNSTGKLMLQILASFSEFERNTILENIYTGQHQRALEGYYQGNL
PLGYNNIPDNKKELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGNPFSISAVTYILSNPFYIGKIQFAKY
KDWNDKRRKGLNDKPVIAEGKHSPIIGKDLWDKVQARKKQVSKKPQVHGKGTNLLTGIIFCEKCGAAYAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIIKSDKVLKQVVERVNQENQIDIAALNHDIAYKQQQFDE
INTKLKNLIQTIEDNPDLTSALKPTIHQYETQLNDITNQMNQLKHQQNQEKPSYDTKQIAALLQQIFQNIESMDKAQLKA
LYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1626 bp        

>NTDB_id=1058110 ACFMSI_RS01490 WP_381543575.1 337185..338810(+) (ccrB) [Staphylococcus aureus strain JB2E56]
ATGGATAAAATGAAGAAAAAGCTTGTAGGAGGCTACATTCGCGTATCCACAGAGAGACAAGTAGAGGGTTATAGCATCGA
GGGACAAATTACACAAATAGAGCAATATTGCCAATTTAACAGCTATGAACTCGTTGATATATATGCAGATCGGGGTATAT
CAGGAAAATCTATGAACCGTCCTGAATTACAGCGCATGTTAAATGATGCTAAAAATGGAAAATTAGACTGTGTTATGGTT
TATAAAACAAATCGTTTGGCACGTAATACTTCCGATTTACTTACAATCGTCGAAGAACTTCATCGTCAAAATGTTGAATT
TTTTAGCTTGTCTGAACGCATGGAAGTCAAAAATTCAACAGGTAAGTTAATGCTCCAGATACTTGCAAGTTTTTCCGAAT
TTGAAAGAAATACAATTTTAGAGAACATTTACACTGGACAACATCAAAGAGCTTTAGAGGGCTATTATCAAGGCAATCTT
CCATTAGGATATAATAACATACCTGATAATAAAAAAGAATTAATGATTAATCAACATGAAGCTAACATCGTTAAATATAT
CTTTGAATCTTATGCCAAAGGTCATGGTTATCGTAAAATAGCCAATGCACTCAATCATAAAGGTTATGTCACTAAGAAAG
GTAATCCATTTAGTATTTCAGCTGTTACTTATATTCTCTCAAACCCATTCTATATTGGTAAAATTCAATTCGCGAAATAC
AAAGATTGGAATGATAAACGTCGTAAAGGCTTGAATGATAAGCCAGTAATCGCTGAAGGTAAGCACTCCCCTATTATTGG
CAAAGACTTATGGGATAAAGTCCAAGCACGTAAGAAACAAGTAAGCAAAAAGCCACAAGTACATGGTAAAGGGACAAATC
TGTTAACTGGAATAATTTTTTGTGAGAAATGCGGCGCGGCATATGCAGCTTCAAATACAACCAATACACTCAAAGACGGT
ACTAAAAAACGCATTCGGTATTATTCATGTAGTAACTTTCGCAATAAAGGTTCAAAAGTGTGTTCAGCCAATAGTGTTAG
AGCTGATGTTATCGAAAAATATGTTATGGATCAAATACTCGAAATTATCAAAAGCGATAAAGTTCTCAAGCAAGTTGTCG
AACGCGTCAATCAAGAGAATCAAATCGATATAGCTGCACTTAACCACGATATCGCTTATAAACAACAACAATTTGATGAA
ATAAATACTAAACTAAAAAATCTAATTCAAACCATCGAAGACAATCCAGACTTAACATCTGCACTCAAACCAACCATTCA
TCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAGCACCAACAAAATCAAGAGAAACCATCTT
ATGATACGAAACAAATCGCTGCCCTATTACAACAAATATTTCAAAACATAGAATCAATGGATAAAGCACAACTCAAAGCA
TTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTCAAACT
CAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTTTGC
CTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-154)

Recombinase (Recombinase)

(179-279)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(294-359)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

79.889

100

0.8

Multiple sequence alignment