Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACFBML_RS14220 Genome accession   NZ_CP170491
Coordinates   3108899..3109474 (-) Length   191 a.a.
NCBI ID   WP_005451499.1    Uniprot ID   A0A9X3MLM3
Organism   Vibrio harveyi strain LcV6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3103899..3114474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFBML_RS14195 (ACFBML_14195) aroB 3104182..3105282 (-) 1101 WP_009697563.1 3-dehydroquinate synthase -
  ACFBML_RS14200 (ACFBML_14200) aroK 3105314..3105832 (-) 519 WP_390338159.1 shikimate kinase AroK -
  ACFBML_RS14205 (ACFBML_14205) pilQ 3106022..3107773 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  ACFBML_RS14210 (ACFBML_14210) pilP 3107808..3108323 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  ACFBML_RS14215 (ACFBML_14215) pilO 3108313..3108906 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFBML_RS14220 (ACFBML_14220) pilN 3108899..3109474 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  ACFBML_RS14225 (ACFBML_14225) pilM 3109458..3110474 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene
  ACFBML_RS14230 (ACFBML_14230) - 3110653..3113223 (+) 2571 WP_050902270.1 penicillin-binding protein 1A -
  ACFBML_RS14235 (ACFBML_14235) oxyR 3113290..3114198 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22244.63 Da        Isoelectric Point: 9.1586

>NTDB_id=1057702 ACFBML_RS14220 WP_005451499.1 3108899..3109474(-) (pilN) [Vibrio harveyi strain LcV6]
MLYHINLLAWRENQREEHRRRFLSLIVLGVIVALGVQWGIGKFYQYQQDQQQQRLDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKIVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTPRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASVKGGNQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=1057702 ACFBML_RS14220 WP_005451499.1 3108899..3109474(-) (pilN) [Vibrio harveyi strain LcV6]
ATGCTGTATCACATTAACCTCCTTGCGTGGCGTGAGAATCAACGTGAAGAACACCGCCGCCGCTTTCTCAGTTTGATCGT
CTTGGGGGTGATTGTTGCGTTGGGCGTGCAATGGGGCATTGGTAAGTTCTATCAATATCAGCAGGATCAACAACAGCAGC
GCCTTGATTATTTGGCGCACTACATCTCCCAGCTTGATAAACGAATCGAAGCGATGAAGATTGCTGAACAAGAGCACAGC
AAGATCTTGGAGAGGTTAAAGATCGTTGAAGGTTTGCAAAATGGGCGTAATAAGACCACGGAGTTCATGAACCTGATGCC
GTCGGTGATCCCTGAAGGAGTCTACGTCGATAAGATCAAAATGAATGATTATGAAATTGAAATCTCGGGTATCTCTGATA
GTACACCTCGCTTGGCCACCATGCTCGACAACATGGAGCGTTCAGCCAAGCTGCTAGATGTCGACATGCATTCTATCGTT
CATGGTAAAGCGCGTTTTGGGAAAGAGTTTCAGACTTTCAAAGTTTCCTTCATGTTCAAAGACGCCTCGGTGAAAGGAGG
TAACCAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

94.241

100

0.942
  pilN Vibrio cholerae strain A1552

60.452

92.67

0.56

Multiple sequence alignment