Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACFBML_RS14225 Genome accession   NZ_CP170491
Coordinates   3109458..3110474 (-) Length   338 a.a.
NCBI ID   WP_026000383.1    Uniprot ID   A0A3A1Q0R6
Organism   Vibrio harveyi strain LcV6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3104458..3115474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFBML_RS14200 (ACFBML_14200) aroK 3105314..3105832 (-) 519 WP_390338159.1 shikimate kinase AroK -
  ACFBML_RS14205 (ACFBML_14205) pilQ 3106022..3107773 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  ACFBML_RS14210 (ACFBML_14210) pilP 3107808..3108323 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  ACFBML_RS14215 (ACFBML_14215) pilO 3108313..3108906 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFBML_RS14220 (ACFBML_14220) pilN 3108899..3109474 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  ACFBML_RS14225 (ACFBML_14225) pilM 3109458..3110474 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene
  ACFBML_RS14230 (ACFBML_14230) - 3110653..3113223 (+) 2571 WP_050902270.1 penicillin-binding protein 1A -
  ACFBML_RS14235 (ACFBML_14235) oxyR 3113290..3114198 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  ACFBML_RS14240 (ACFBML_14240) - 3114373..3115101 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38066.43 Da        Isoelectric Point: 6.4206

>NTDB_id=1057703 ACFBML_RS14225 WP_026000383.1 3109458..3110474(-) (pilM) [Vibrio harveyi strain LcV6]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDSQVATEQFN
RQIVDRVKRQIQLYTSINGNQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1057703 ACFBML_RS14225 WP_026000383.1 3109458..3110474(-) (pilM) [Vibrio harveyi strain LcV6]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAACAAGACCCTCTTGCTGAAGACTCGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAATCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A1Q0R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment