Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACFIOO_RS07310 Genome accession   NZ_CP170406
Coordinates   1539638..1540135 (-) Length   165 a.a.
NCBI ID   WP_077446653.1    Uniprot ID   -
Organism   Staphylococcus aureus strain t21635     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1534638..1545135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOO_RS07285 gcvPB 1534800..1536272 (-) 1473 WP_000202180.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACFIOO_RS07290 gcvPA 1536265..1537611 (-) 1347 WP_054190021.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACFIOO_RS07295 gcvT 1537631..1538722 (-) 1092 WP_054190022.1 glycine cleavage system aminomethyltransferase GcvT -
  ACFIOO_RS07300 - 1538881..1539405 (-) 525 WP_054190023.1 shikimate kinase -
  ACFIOO_RS07305 - 1539395..1539541 (-) 147 WP_001789879.1 hypothetical protein -
  ACFIOO_RS07310 comGF 1539638..1540135 (-) 498 WP_077446653.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACFIOO_RS07315 comGE 1540053..1540352 (-) 300 WP_054190024.1 hypothetical protein Machinery gene
  ACFIOO_RS07320 comGD 1540339..1540785 (-) 447 WP_077446655.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFIOO_RS07325 comGC 1540763..1541074 (-) 312 WP_054190026.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFIOO_RS07330 comGB 1541088..1542158 (-) 1071 WP_054190027.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFIOO_RS07335 comGA 1542130..1543104 (-) 975 WP_054191886.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFIOO_RS07340 - 1543156..1543779 (-) 624 WP_054190028.1 MBL fold metallo-hydrolase -
  ACFIOO_RS07345 - 1543776..1544105 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACFIOO_RS07350 - 1544105..1545091 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19129.87 Da        Isoelectric Point: 9.9584

>NTDB_id=1056848 ACFIOO_RS07310 WP_077446653.1 1539638..1540135(-) (comGF) [Staphylococcus aureus strain t21635]
MILSKVTNKFVLLQKIPLLIKRHVYSINVKAFSLVEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRHQRIILHKGEEMIEYKLLNNKIIKVVNDEGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1056848 ACFIOO_RS07310 WP_077446653.1 1539638..1540135(-) (comGF) [Staphylococcus aureus strain t21635]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTACTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAGTTGAAATGTTAGTAGCGATGATGGTCATAAGTATAATTTTACTAATTGTTCCAGATT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCATCAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTATTGAATAATAAAATTATTAAAGTTGTAAATGACGAGGGTAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

95.758

100

0.958
  comGF Staphylococcus aureus MW2

94.545

100

0.945

Multiple sequence alignment