Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACFIOB_RS07315 Genome accession   NZ_CP170401
Coordinates   1538390..1538887 (-) Length   165 a.a.
NCBI ID   WP_029694423.1    Uniprot ID   -
Organism   Staphylococcus aureus strain t10543     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1533390..1543887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOB_RS07290 gcvPB 1533552..1535024 (-) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACFIOB_RS07295 gcvPA 1535017..1536363 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACFIOB_RS07300 gcvT 1536383..1537474 (-) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  ACFIOB_RS07305 - 1537633..1538157 (-) 525 WP_001015120.1 shikimate kinase -
  ACFIOB_RS07310 - 1538147..1538293 (-) 147 WP_001789879.1 hypothetical protein -
  ACFIOB_RS07315 comGF 1538390..1538887 (-) 498 WP_029694423.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACFIOB_RS07320 comGE 1538823..1539104 (-) 282 WP_000844412.1 hypothetical protein Machinery gene
  ACFIOB_RS07325 comGD 1539091..1539537 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFIOB_RS07330 comGC 1539515..1539826 (-) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFIOB_RS07335 comGB 1539840..1540910 (-) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFIOB_RS07340 comGA 1540882..1541856 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFIOB_RS07345 - 1541908..1542531 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  ACFIOB_RS07350 - 1542528..1542857 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACFIOB_RS07355 - 1542857..1543843 (-) 987 WP_000161307.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19155.82 Da        Isoelectric Point: 10.0355

>NTDB_id=1056766 ACFIOB_RS07315 WP_029694423.1 1538390..1538887(-) (comGF) [Staphylococcus aureus strain t10543]
MILSKVTNKYVPFQKIPLLINRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1056766 ACFIOB_RS07315 WP_029694423.1 1538390..1538887(-) (comGF) [Staphylococcus aureus strain t10543]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAATAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

95.152

100

0.952

Multiple sequence alignment