Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACFIOB_RS07340 Genome accession   NZ_CP170401
Coordinates   1540882..1541856 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain t10543     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1535882..1546856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOB_RS07300 gcvT 1536383..1537474 (-) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  ACFIOB_RS07305 - 1537633..1538157 (-) 525 WP_001015120.1 shikimate kinase -
  ACFIOB_RS07310 - 1538147..1538293 (-) 147 WP_001789879.1 hypothetical protein -
  ACFIOB_RS07315 comGF 1538390..1538887 (-) 498 WP_029694423.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACFIOB_RS07320 comGE 1538823..1539104 (-) 282 WP_000844412.1 hypothetical protein Machinery gene
  ACFIOB_RS07325 comGD 1539091..1539537 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFIOB_RS07330 comGC 1539515..1539826 (-) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFIOB_RS07335 comGB 1539840..1540910 (-) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFIOB_RS07340 comGA 1540882..1541856 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFIOB_RS07345 - 1541908..1542531 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  ACFIOB_RS07350 - 1542528..1542857 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACFIOB_RS07355 - 1542857..1543843 (-) 987 WP_000161307.1 ROK family glucokinase -
  ACFIOB_RS07360 - 1543840..1544043 (-) 204 WP_000087561.1 YqgQ family protein -
  ACFIOB_RS07365 - 1544024..1545487 (-) 1464 WP_001017870.1 rhomboid family protein -
  ACFIOB_RS07370 - 1545499..1546038 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  ACFIOB_RS07375 rpmG 1546247..1546396 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=1056771 ACFIOB_RS07340 WP_000697220.1 1540882..1541856(-) (comGA) [Staphylococcus aureus strain t10543]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1056771 ACFIOB_RS07340 WP_000697220.1 1540882..1541856(-) (comGA) [Staphylococcus aureus strain t10543]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTGATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment