Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   BCU28_RS15530 Genome accession   NZ_CP170022
Coordinates   3423536..3424150 (-) Length   204 a.a.
NCBI ID   WP_010435288.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.45.B7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3418536..3429150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU28_RS15505 (BCU28_15505) aroB 3418850..3419938 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  BCU28_RS15510 (BCU28_15510) aroK 3419963..3420481 (-) 519 WP_009848907.1 shikimate kinase AroK -
  BCU28_RS15515 (BCU28_15515) pilQ 3420658..3422349 (-) 1692 WP_016787694.1 type IV pilus secretin PilQ Machinery gene
  BCU28_RS15520 (BCU28_15520) pilP 3422409..3422927 (-) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  BCU28_RS15525 (BCU28_15525) pilO 3422914..3423543 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  BCU28_RS15530 (BCU28_15530) pilN 3423536..3424150 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  BCU28_RS15535 (BCU28_15535) pilM 3424134..3425159 (-) 1026 WP_016790839.1 type IV pilus assembly protein PilM Machinery gene
  BCU28_RS15540 (BCU28_15540) - 3425311..3427869 (+) 2559 WP_010435294.1 penicillin-binding protein 1A -
  BCU28_RS15545 (BCU28_15545) oxyR 3427965..3428858 (-) 894 WP_016790838.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23345.82 Da        Isoelectric Point: 9.3072

>NTDB_id=1054714 BCU28_RS15530 WP_010435288.1 3423536..3424150(-) (pilN) [Vibrio cyclitrophicus strain 10N.286.45.B7]
MLHQINLLSWRDEIRAQHKKRFIHLVILGVIIALAGQWAVGHYFHDQQAKQQARLSYLDKYIAVLDRQIQSLKVAEQEHK
AILTRLAVVESLQFGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKEVEMHSIV
HNRKRFNKEFQSFKVSFVFSPVSLENTTAEGTTANIKEKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1054714 BCU28_RS15530 WP_010435288.1 3423536..3424150(-) (pilN) [Vibrio cyclitrophicus strain 10N.286.45.B7]
ATGTTGCATCAAATTAACCTGTTGTCGTGGCGTGATGAGATTCGCGCTCAGCATAAAAAGCGTTTCATTCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTGCCGGGCAATGGGCGGTGGGACACTATTTTCATGACCAACAAGCCAAGCAGCAAGCGC
GCCTCAGTTATCTCGATAAGTACATCGCAGTGCTCGACCGACAAATCCAGTCATTAAAAGTCGCAGAGCAAGAGCACAAA
GCCATCCTTACACGACTTGCTGTCGTGGAGTCTTTACAGTTCGGGCGCAATAAAACCACCGACTTTATGAACCTGATGCC
GGAGCTGATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GCACCGCTCGTCTAGCCACCATGTTGGACAACCTAGAACGTTCTGACTCTCTTAAAGAAGTTGAGATGCACTCTATTGTT
CATAACCGTAAGCGCTTCAATAAAGAGTTTCAGAGCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTTTCTTTAGAAAACAC
AACCGCAGAGGGCACAACTGCGAACATAAAAGAGAAGGGGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.731

94.608

0.603
  pilN Vibrio campbellii strain DS40M4

61.78

93.627

0.578

Multiple sequence alignment