Detailed information NTG1054715

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BCU28_RS15535
• Genome accession: NZ_CP170022
• Coordinates: 3424134..3425159 (-)
• Length: 341 a.a.
• NCBI ID: WP_016790839.1
• Uniprot ID: -
• Organism: Vibrio cyclitrophicus strain 10N.286.45.B7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3419134..3430159

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCU28_RS15510 (BCU28_15510)	aroK	3419963..3420481 (-)	519	WP_009848907.1	shikimate kinase AroK	-
BCU28_RS15515 (BCU28_15515)	pilQ	3420658..3422349 (-)	1692	WP_016787694.1	type IV pilus secretin PilQ	Machinery gene
BCU28_RS15520 (BCU28_15520)	pilP	3422409..3422927 (-)	519	WP_016787693.1	pilus assembly protein PilP	Machinery gene
BCU28_RS15525 (BCU28_15525)	pilO	3422914..3423543 (-)	630	WP_010435284.1	type 4a pilus biogenesis protein PilO	Machinery gene
BCU28_RS15530 (BCU28_15530)	pilN	3423536..3424150 (-)	615	WP_010435288.1	PilN domain-containing protein	Machinery gene
BCU28_RS15535 (BCU28_15535)	pilM	3424134..3425159 (-)	1026	WP_016790839.1	type IV pilus assembly protein PilM	Machinery gene
BCU28_RS15540 (BCU28_15540)	-	3425311..3427869 (+)	2559	WP_010435294.1	penicillin-binding protein 1A	-
BCU28_RS15545 (BCU28_15545)	oxyR	3427965..3428858 (-)	894	WP_016790838.1	DNA-binding transcriptional regulator OxyR	-
BCU28_RS15550 (BCU28_15550)	-	3429080..3429808 (+)	729	WP_010435300.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38127.79 Da
• Isoelectric Point: 7.0275

>NTDB_id=1054715 BCU28_RS15535 WP_016790839.1 3424134..3425159(-) (pilM) [Vibrio cyclitrophicus strain 10N.286.45.B7]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMNMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=1054715 BCU28_RS15535 WP_016790839.1 3424134..3425159(-) (pilM) [Vibrio cyclitrophicus strain 10N.286.45.B7]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGATCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGAATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	53.687	99.413	0.534
pilM	Vibrio campbellii strain DS40M4	47.661	100	0.478