Detailed information NTG1052159

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: ACE2AN_RS00200
• Genome accession: NZ_CP169564
• Coordinates: 30862..32223 (+)
• Length: 453 a.a.
• NCBI ID: WP_074017595.1
• Uniprot ID: A0A237IXT5
• Organism: Streptococcus pneumoniae strain 6_2F1
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	12174..39052	30862..32223	within	0

Gene organization within MGE regions

Location: 12174..39052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE2AN_RS00065	ftsH	12174..14132 (+)	1959	WP_000744557.1	ATP-dependent zinc metalloprotease FtsH	-
ACE2AN_RS00070	comX/comX2	14254..14733 (+)	480	WP_000588897.1	sigma-70 family RNA polymerase sigma factor	Regulator
ACE2AN_RS00105	-	20283..20492 (+)	210	Protein_14	transposase	-
ACE2AN_RS00110	-	20527..21324 (-)	798	Protein_15	transposase	-
ACE2AN_RS00115	comW	21590..21826 (+)	237	WP_000939546.1	sigma(X)-activator ComW	Regulator
ACE2AN_RS00120	-	22057..23343 (+)	1287	WP_000205044.1	adenylosuccinate synthase	-
ACE2AN_RS00125	tadA	23544..24011 (+)	468	WP_000291875.1	tRNA adenosine(34) deaminase TadA	-
ACE2AN_RS00135	-	24220..25356 (-)	1137	WP_001824497.1	site-specific integrase	-
ACE2AN_RS00140	-	25417..26487 (-)	1071	WP_000401841.1	type I restriction endonuclease	-
ACE2AN_RS00145	-	26504..26884 (-)	381	WP_081528400.1	ImmA/IrrE family metallo-endopeptidase	-
ACE2AN_RS00150	-	26897..27160 (-)	264	WP_000285962.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ACE2AN_RS00155	-	27160..27393 (-)	234	WP_000156419.1	hypothetical protein	-
ACE2AN_RS00160	-	27393..27761 (-)	369	WP_000464160.1	helix-turn-helix domain-containing protein	-
ACE2AN_RS00165	-	28175..28414 (-)	240	WP_000142893.1	hypothetical protein	-
ACE2AN_RS00170	-	28479..28670 (+)	192	WP_001112859.1	DNA-binding protein	-
ACE2AN_RS00175	-	28693..28896 (+)	204	WP_001247549.1	hypothetical protein	-
ACE2AN_RS00180	-	29051..29218 (-)	168	WP_000024181.1	YjzC family protein	-
ACE2AN_RS00185	-	29223..29603 (+)	381	Protein_29	autolysin	-
ACE2AN_RS00190	-	29888..30331 (+)	444	WP_000701992.1	dUTP diphosphatase	-
ACE2AN_RS00195	-	30333..30848 (+)	516	WP_061372761.1	histidine phosphatase family protein	-
ACE2AN_RS00200	radA	30862..32223 (+)	1362	WP_074017595.1	DNA repair protein RadA	Machinery gene
ACE2AN_RS00205	-	32296..32793 (+)	498	WP_001809263.1	carbonic anhydrase	-
ACE2AN_RS00210	-	32818..33601 (+)	784	Protein_34	PrsW family glutamic-type intramembrane protease	-
ACE2AN_RS00215	-	33746..34714 (+)	969	WP_000010163.1	ribose-phosphate diphosphokinase	-
ACE2AN_RS00220	-	34848..35129 (-)	282	Protein_36	transposase family protein	-
ACE2AN_RS00225	-	35256..36163 (-)	908	Protein_37	Rpn family recombination-promoting nuclease/putative transposase	-
ACE2AN_RS00230	polA	36419..39052 (+)	2634	WP_024478011.1	DNA polymerase I	-

Sequence

Protein

• Length: 453 a.a.
• Molecular weight: 49432.95 Da
• Isoelectric Point: 6.5137

>NTDB_id=1052159 ACE2AN_RS00200 WP_074017595.1 30862..32223(+) (radA) [Streptococcus pneumoniae strain 6_2F1]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide

• Length: 1362 bp

>NTDB_id=1052159 ACE2AN_RS00200 WP_074017595.1 30862..32223(+) (radA) [Streptococcus pneumoniae strain 6_2F1]
ATCGCAAAGAAAAAAGCGACATTTGTTTGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTATCCTTGACAGGTGAGAAAA
CTAAGCCTATGAAACTGGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (63-218)

• Subunit ChlI of Mg-chelatase (ChlI) (360-425)

3D structure

• PDB: 8RXC

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus pneumoniae Rx1	100	100	1
radA	Streptococcus pneumoniae D39	100	100	1
radA	Streptococcus pneumoniae R6	100	100	1
radA	Streptococcus pneumoniae TIGR4	100	100	1
radA	Streptococcus mitis NCTC 12261	99.558	100	0.996
radA	Streptococcus mitis SK321	99.558	100	0.996
radA	Bacillus subtilis subsp. subtilis str. 168	63.135	100	0.631